Anti-CRM1 [EPR14124] antibody - C-terminal

• Supplier: Abcam
• Catalog: ab191081
• Prices: $384.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR14124
• Applications: IHC-P FC ICC/IF ICC/IF WB
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CRM1 aa 1000 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: XPO1
• Conjugate: Unconjugated

Product images

Flow cytometric analysis of K562 cells (paraformaldehyde-fixed, 2%) labeling CRM1 with ab191081 at 1/160 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed; 0.1% tritonX-100-permeabilized) labeling CRM1 with ab191081 at 1/50 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

Immunohistochemical analysis of paraffin-embedded Human bladder transitional cell carcinoma tissue labeling CRM1 with ab191081 at 1/50 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin (inset: negative control).

All lanes : Anti-CRM1 [EPR14124] antibody - C-terminal (ab191081) at 1/5000 dilutionLane 1 : C6 cell lysateLane 2 : PC12 cell lysateLane 3 : NIH 3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugate at 1/1000 dilution

All lanes : Anti-CRM1 [EPR14124] antibody - C-terminal (ab191081) at 1/20000 dilutionLane 1 : K562 cell lysateLane 2 : HeLa cell lysateLane 3 : Jurkat cell lysateLane 4 : Ramos cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugate at 1/1000 dilution