Anti-CROT antibody
| Name | Anti-CROT antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab103448 |
| Prices | $370.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | FC WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide corresponding to a region within N terminal amino acids 57-86 of Human CROT, conjugated to KLH |
| Description | Rabbit Polyclonal |
| Gene | CROT |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab103448 stained T24/83 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum/ 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab103448 at 5 µg/mL overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti-Rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-CROT antibody (ab103448) at 1/50 dilutionLane 1 : K562 cell lysateLane 2 : Mouse liver tissue lysateLysates/proteins at 35 µg per lane.
ab103448, at a 1/50 dilution, staining CROT in formalin fixed and paraffin embedded Human brain tissue which was peroxidase conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of K562 cells with ab103448 at a 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.