CROT

Gene Symbol CROT
Entrez Gene 54677
Alt Symbol COT
Species Human
Gene Type protein-coding
Description carnitine O-octanoyltransferase
Other Description peroxisomal carnitine O-octanoyltransferase|peroxisomal carnitine acyltransferase
Swissprots Q9Y6I2 A4D1D6 E7EQF2 Q86V17 Q9UKG9 Q8IUW9
Accessions EAL24177 EAW76954 Q9UKG9 AA062685 AF052187 AF073770 AAD41654 AF168793 AAF03234 AK127901 AK300114 BAG61909 AK313487 BC006015 BC012483 BC019226 BC039004 AAH39004 BC051874 AAH51874 DA518578 DA865171 XM_011516337 XP_011514639 NM_001143935 NP_001137407 NM_001243745 NP_001230674 NM_021151 NP_066974
Function Beta-oxidation of fatty acids. The highest activity concerns the C6 to C10 chain length substrate. Converts the end product of pristanic acid beta oxidation, 4,8-dimethylnonanoyl- CoA, to its corresponding carnitine ester. {ECO:0000269|PubMed:10486279}.
Subcellular Location Peroxisome {ECO:0000305}.
Top Pathways Peroxisome

CROT (E-2) - sc-365408 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CROT (E-2): sc-365408. Western blot analysis of CROT expression in A-431 whole cell lysate.

CROT (N-15) - sc-162715 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CROT (N-15): sc-162715. Immunoperoxidase staining of formalin fixed, paraffin-embedded human small instestine tissue showing cytoplasmic staining of glandular cells.

CROT (H-1) - sc-365976 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CROT (H-1): sc-365976. Western blot analysis of CROT expression in A-431 (A) and RAW 264.7 (B) whole cell lysates.

CROT (H-300) - sc-134608 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
CROT (H-300): sc-134608. Western blot analysis of CROT expression in non-transfected: sc-117752 (A) and human CROT transfected: sc-115494 (B) 293T whole cell lysates.

CROT (V-15) - sc-162717 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-CROT antibody - ab103448 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab103448 stained T24/83 cells.  The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum/ 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab103448 at 5 µg/mL overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti-Rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-CROT antibody - ab95953 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-CROT antibody (ab95953) at 1/1000 dilutionLane 1 : 293T whole cell lysateLane 2 : A431 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-CROT antibody - ab57405 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-COT / CROT Antibody (aa2-100, clone 10B2603, Azide-free) - LS-C183502 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone 10B2603

Anti-COT / CROT Antibody (C-Terminus) - LS-C139743 from LifeSpan Bioscience

Host Goat
Clonality Polyclonal
CROT antibody (0.3 ug/ml) staining of Rat Kidney lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-COT / CROT Antibody (N-Terminus) - LS-C81310 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 2.5 ug/ml. Gel concentration: 12%.

Anti-COT / CROT Antibody (aa2-100) - LS-C307095 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-COT / CROT Antibody - LS-C309997 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-COT / CROT Antibody (aa178-199) - LS-C186472 from LifeSpan Bioscience

Host Goat
Clonality Polyclonal
CROT antibody (1 ug/ml) staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-COT / CROT Antibody (phospho-Thr290) - LS-C192154 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
Immunohistochemistry (IHC) analysis of p-Cot (T290) pAb in paraffin-embedded human brain tissue.

Anti-COT / CROT Antibody (aa57-86) - LS-C167055 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human brain tissue reacted with CROT Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Anti-MAP3K8 / TPL2 Antibody (Pro284) - LS-C175974 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
IHC of COT (P284) pAb in paraffin-embedded human brain tissue.

Anti-COT / CROT Antibody (aa140-462) - LS-C185696 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded Ca922 xenograft using CROT antibody at 1:100 dilution.

CROT Antibody - NBP1-31441 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

CROT Antibody - NBP1-85501 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: CROT Antibody [NBP1-85501] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp

CROT Antibody (1A6) - H00054677-M01 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1A6
Western Blot: CROT Antibody (1A6) [H00054677-M01] - CROT monoclonal antibody (M01), clone 1A6. Analysis of CROT expression in Raw 264.7.

CROT Antibody - NBP1-85503 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: CROT Antibody [NBP1-85503] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

CROT Antibody - NBP1-85502 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

CROT Antibody - H00054677-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: CROT Antibody [H00054677-B01] - analysis of CROT expression in transfected 293T cell line by CROT MaxPab polyclonal antibody (B01). Lane 1: CROT transfected lysate (70.20 KDa). Lane 2: Non-transfected lysate.

CROT Antibody - H00054677-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: CROT Antibody [H00054677-A01] - CROT polyclonal antibody (A01), Lot # 051205JC01 Western Blot analysis of CROT expression in MES-SA/Dx5 ( Cat # L021V1 ).
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