Anti-Cryptochrome I antibody
| Name | Anti-Cryptochrome I antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab54649 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | WB ICC/IF ICC/IF IHC-P FC |
| Species Reactivities | Human |
| Antigen | Recombinant full length protein, corresponding to amino acids 1-587 of Human Cryptochrome I |
| Description | Mouse Monoclonal |
| Gene | CRY1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Cryptochrome I antibody (ab54649) used in immunofluorescence at 10ug/ml on HeLa cells.
Cryptochrome I antibody (ab54649) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human colon adenocarcinoma tissue.
![Overlay histogram showing HeLa cells stained with ab54649 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54649, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/2623_Cryptochrome-I-Primary-antibodies-ab54649-3.jpg)
Overlay histogram showing HeLa cells stained with ab54649 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54649, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.