All lanes : Anti-CSK antibody [5F3] (ab105930) at 1/500 dilutionLane 1 : NIH/3T3 cell lysate.Lane 2 : Hela cell lysate.Lane 3 : COS7 cell lysate.Lane 4 : Jurkat cell lysate.Lane 5 : Raw246.7 cell lysate.Lane 6 : A549 cell lysate.Lane 7 : HL-60 cell lysate.Lane 8 : PC-12 cell lysate.
Immunofluorescence analysis of U251 cells using ab105930 (green) at 1/200 dilution. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric analysis of HL-60 cells using ab105930 (green) and negative control (purple).