Anti-Ctip1 antibody [14B5]
| Name | Anti-Ctip1 antibody [14B5] |
|---|---|
| Supplier | Abcam |
| Catalog | ab19487 |
| Prices | $392.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 14B5 |
| Applications | ICC/IF ICC/IF IHC-P WB ICC/IF IP IHC-P ChIP FC |
| Species Reactivities | Mouse, Human, Zebrafish |
| Antigen | A cocktail of BCL11A fusion proteins |
| Description | Mouse Monoclonal |
| Gene | BCL11A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing Ramos cells stained with ab19487 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab19487, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/3096_ab19487-4-ab19487FC.jpg)
Overlay histogram showing Ramos cells stained with ab19487 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab19487, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![All lanes : Anti-Ctip1 antibody [14B5] (ab19487)Lane 1 : C6 Cell LineLane 2 : MEF Cell LineLane 3 : HDFn Cell LIneLane 4 : Jurkat Cell LineLane 5 : HEK293T Cell LineLysates/proteins at 20 µg per lane.developed using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/3097_ab19487-195198-ab19487Figure-3.jpg)
All lanes : Anti-Ctip1 antibody [14B5] (ab19487)Lane 1 : C6 Cell LineLane 2 : MEF Cell LineLane 3 : HDFn Cell LIneLane 4 : Jurkat Cell LineLane 5 : HEK293T Cell LineLysates/proteins at 20 µg per lane.developed using the ECL techniquePerformed under reducing conditions.
![Anti-Ctip1 antibody [14B5] (ab19487) at 1 µg/ml + Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate at 10 µgSecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/3098_Ctip1-Primary-antibodies-ab19487-4.jpg)
Anti-Ctip1 antibody [14B5] (ab19487) at 1 µg/ml + Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate at 10 µgSecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
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In vivo localization and identification of SUMOylated proteins in the brain of - In vivo localization and identification of SUMOylated proteins in the brain of
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Binding patterns of BCL11A in the globin and GATA1 loci and characterization of - Binding patterns of BCL11A in the globin and GATA1 loci and characterization of
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Developmental and species-divergent globin switching are driven by BCL11A. - Developmental and species-divergent globin switching are driven by BCL11A.
Sankaran VG, Xu J, Ragoczy T, Ippolito GC, Walkley CR, Maika SD, Fujiwara Y, Ito M, Groudine M, Bender MA, Tucker PW, Orkin SH. Nature. 2009 Aug 27;460(7259):1093-7.
Human fetal hemoglobin expression is regulated by the developmental - Human fetal hemoglobin expression is regulated by the developmental
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The BCL11AXL transcription factor: its distribution in normal and malignant - The BCL11AXL transcription factor: its distribution in normal and malignant
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BCL11A-dependent recruitment of SIRT1 to a promoter template in mammalian cells - BCL11A-dependent recruitment of SIRT1 to a promoter template in mammalian cells
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