Anti-Ctip1 antibody [18B12DE6] - ChIP Grade
| Name | Anti-Ctip1 antibody [18B12DE6] - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab19489 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 18B12DE6 |
| Applications | ICC/IF ICC/IF IHC-P WB ICC/IF IP FC ChIP IHC-F |
| Species Reactivities | Mouse, Human |
| Antigen | A cocktail of BCL11A fusion proteins |
| Description | Mouse Monoclonal |
| Gene | BCL11A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Human normal tonsil. Staining is localised to cytoplasm and nuclei. Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for mouse for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
![Overlay histogram showing Ramos cells stained with ab19489 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab19489, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Ramos cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/3101_Ctip1-Primary-antibodies-ab19489-3.jpg)
Overlay histogram showing Ramos cells stained with ab19489 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab19489, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Ramos cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
A signature motif mediating selective interactions of BCL11A with the NR2E/F - A signature motif mediating selective interactions of BCL11A with the NR2E/F
Chan CM, Fulton J, Montiel-Duarte C, Collins HM, Bharti N, Wadelin FR, Moran PM, Mongan NP, Heery DM. Nucleic Acids Res. 2013 Nov;41(21):9663-79.
A global DNA methylation and gene expression analysis of early human B-cell - A global DNA methylation and gene expression analysis of early human B-cell
Lee ST, Xiao Y, Muench MO, Xiao J, Fomin ME, Wiencke JK, Zheng S, Dou X, de Smith A, Chokkalingam A, Buffler P, Ma X, Wiemels JL. Nucleic Acids Res. 2012 Dec;40(22):11339-51.
Binding patterns of BCL11A in the globin and GATA1 loci and characterization of - Binding patterns of BCL11A in the globin and GATA1 loci and characterization of
Jawaid K, Wahlberg K, Thein SL, Best S. Blood Cells Mol Dis. 2010 Aug 15;45(2):140-6.
Functional studies of BCL11A: characterization of the conserved BCL11A-XL splice - Functional studies of BCL11A: characterization of the conserved BCL11A-XL splice
Liu H, Ippolito GC, Wall JK, Niu T, Probst L, Lee BS, Pulford K, Banham AH, Stockwin L, Shaffer AL, Staudt LM, Das C, Dyer MJ, Tucker PW. Mol Cancer. 2006 May 16;5:18.
The BCL11AXL transcription factor: its distribution in normal and malignant - The BCL11AXL transcription factor: its distribution in normal and malignant
Pulford K, Banham AH, Lyne L, Jones M, Ippolito GC, Liu H, Tucker PW, Roncador G, Lucas E, Ashe S, Stockwin L, Walewska R, Karran L, Gascoyne RD, Mason DY, Dyer MJ. Leukemia. 2006 Aug;20(8):1439-41. Epub 2006 May 18.