Anti-CTLA4 antibody [EPR1476]

• Supplier: Abcam
• Catalog: ab134090
• Prices: $379.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR1476
• Applications: WB ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide corresponding to residues on the N terminus in Human CTLA4 (UniProt P16410)
• Description: Rabbit Monoclonal
• Gene: CTLA4
• Conjugate: Unconjugated

Product images

All lanes : Anti-CTLA4 antibody [EPR1476] (ab134090) at 1/5000 dilution (purified)Lane 1 : Raji cell lysateLane 2 : K562 cell lysateLane 3 : TF-1 cell lysateLysates/proteins at 20 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Anti-CTLA4 antibody [EPR1476] (ab134090) at 1/10000 dilution (purified) + C6 cell lysate at 10 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Anti-CTLA4 antibody [EPR1476] (ab134090) at 1/10000 dilution (purified) + NIH/3T3 cell lysate at 10 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Immunofluorescence staining of Raji cells with purified ab134090 at a working dilution of 1 in 200, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, purified ab134090 was used at a dilution of 1/200 followed by an Alexa Fluor® 594 goat anti-mouse antibody at a dilution of 1/500.

All lanes : Anti-CTLA4 antibody [EPR1476] (ab134090) (unpurified)Lane 1 : Jurkat cell lysateLane 2 : Raji cell lysateLane 3 : Human CTLA4 full length protein (ab69787)