![Overlay histogram showing HeLa cells stained with ab118036 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab118036, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/3338_ab118036-10-ab118036FC.jpg)
Overlay histogram showing HeLa cells stained with ab118036 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab118036, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![All lanes : Anti-CUG-BP1 antibody [5B8] (ab118036) at 1/2000 dilutionLane 1 : HEK293T cells transfected with the pCMV6-ENTRY control Lane 2 : HEK293T cells transfected with the pCMV6-ENTRY CUG-BP1 cDNALysates/proteins at 5 µg per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/3339_CUG-BP1-Primary-antibodies-ab118036-1.jpg)
All lanes : Anti-CUG-BP1 antibody [5B8] (ab118036) at 1/2000 dilutionLane 1 : HEK293T cells transfected with the pCMV6-ENTRY control Lane 2 : HEK293T cells transfected with the pCMV6-ENTRY CUG-BP1 cDNALysates/proteins at 5 µg per lane.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using 1/50 ab118036.
Immunohistochemical staining of paraffin-embedded kidney tissue using 1/50 ab118036.
Immunohistochemical staining of paraffin-embedded liver tissue using 1/50 ab118036.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using 1/50 ab118036.
Immunohistochemical staining of paraffin-embedded pancreas tissue using 1/50 ab118036.
Immunohistochemical staining of paraffin-embedded prostate tissue using 1/50 ab118036.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of prostate tissue using 1/50 ab118036.
ab118036 at 1/100 immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CUG-BP1