All lanes : Anti-Cullin 2 antibody [EPR3104(2)] (ab166917) at 1/1000 dilutionLane 1 : PC3 cell lysateLane 2 : 293T cell lysateLane 3 : HeLa cell lysateLane 4 : Raji cell lysateLysates/proteins at 10 µg per lane.
Immunofluorescent analysis of 293T cells labeling Cullin 2 with ab166917 at 1/100.
Western blot analysis of an immunoprecipitation pellet from Raji cell lysate labeling Cullin 2 with ab166917 which was used at 1/10 dilution for immunoprecipitation.Secondary antibody used was an HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.