Anti-Cullin 3 antibody [EPR3196Y]
| Name | Anti-Cullin 3 antibody [EPR3196Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab75851 |
| Prices | $381.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EPR3196Y |
| Applications | WB IP FC |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | A synthetic peptide corresponding to residues on the C terminus of human Cullin 3 |
| Description | Rabbit Monoclonal |
| Gene | CUL3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Overlay histogram showing HeLa cells stained with ab75851 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75851, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![All lanes : Anti-Cullin 3 antibody [EPR3196Y] (ab75851) at 1/20000 dilutionLane 1 : HeLa cell lysateLane 2 : SH-SY5Y cell lysateLane 3 : PC12 cell lysateLane 4 : 3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP-labeled goat anti-rabbit at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/3401_ab75851wb.jpg)
All lanes : Anti-Cullin 3 antibody [EPR3196Y] (ab75851) at 1/20000 dilutionLane 1 : HeLa cell lysateLane 2 : SH-SY5Y cell lysateLane 3 : PC12 cell lysateLane 4 : 3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP-labeled goat anti-rabbit at 1/2000 dilution