Overlay histogram showing HeLa cells stained with ab92554 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92554, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![All lanes : Anti-Cullin 4a antibody [EPR3198] (ab92554) at 1/20000 dilutionLane 1 : Hela cell lysateLane 2 : A375 cell lysateLane 3 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/3412_Cullin-4a-Primary-antibodies-ab92554-1.jpg)
All lanes : Anti-Cullin 4a antibody [EPR3198] (ab92554) at 1/20000 dilutionLane 1 : Hela cell lysateLane 2 : A375 cell lysateLane 3 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution
ab92554 at 1/100 dilution staining Cullin 4a in Human breast carcinoma by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
ab92554 showing positive staining in Hepatocellular carcinoma tissue.
ab92554 showing positive staining in Colonic adenocarcinoma tissue.
ab92554 showing positive staining in Ovarian carcinoma tissue.