CUL4A

Gene Symbol CUL4A
Entrez Gene 8451
Alt Symbol -
Species Human
Gene Type protein-coding
Description cullin 4A
Other Description CUL-4A|cullin-4A
Swissprots Q9UP17 O75834 Q13619 Q6UP08 A2A2W2 Q589T6 Q5TC62
Accessions EAX09201 Q13619 AB012193 BAA33146 AB178950 BAD93235 AF077188 AAD45191 AK126278 AK296700 BAG59294 AK314835 BAG37354 AL833355 AY365124 AAR13072 BC008308 AAH08308 BC015166 BM835927 BX439434 CA445237 DA478924 DA574202 DB455959 EU176793 ABW03594 U58090 AAC50547 XM_011537523 XP_011535825 XM_011537524 XP_011535826 NM_001008895 NP_001008895 NM_001278513 NP_001265442 NM_001278514 NP_001265443 NM_003589 NP_003580
Function Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. DCX(DDB2) ubiquitinates histones H3-H4 and is required for efficient histone deposition during replication-coupled (H3.1) and replication-independent (H3.3) nucleosome assembly, probably by facilitating the transfer of H3 from ASF1A/ASF1B to other chaperones involved in histone deposition. DCX(DTL) plays a role in PCNA-dep
Top Pathways Ubiquitin mediated proteolysis, Nucleotide excision repair

CUL4A Antibody - 2699 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from COS and SK-MEL-28 cell lines using CUL4A Antibody.

CUL-4A (E-14) - sc-50917 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

CUL-4A (K-16) - sc-50919 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CUL-4A (K-16): sc-50919. Western blot analysis of CUL-4A expression in mouse placenta (A) and mouse lung (B) tissue extracts.

CUL-4A (P-22) - sc-130732 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
CUL-4A (P-22): sc-130732. Western blot analysis of CUL-4A expression in non-transfected (A) and human CUL-4A transfected (B) 293 whole cell lysates.

Anti-Cullin 4a antibody - ab72548 from Abcam

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling Cullin 4a with ab72548 at 1/1000 (1µg/ml). Detection: DAB.

Anti-Cullin 4a antibody - ab167287 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-Cullin 4a antibody (ab167287) at 1 µg/mlLane 1 : Cullin 4a transfected 293T cell line lysateLane 2 : Non-transfected 293T cell line lysateLysates/proteins at 15 µl per lane.

Anti-Cullin 4a antibody - ab137418 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Cullin 4a antibody (ab137418) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

Anti-Cullin 4a antibody - ab84382 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human breast tumor tissue using 1/500 ab84382 followed by DAB staining.

Anti-Cullin 4a antibody - ab72546 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-Cullin 4a antibody [EPR3198] - ab92554 from Abcam

Prices $388.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3198
Overlay histogram showing HeLa cells stained with ab92554 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92554, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-Cullin 4a antibody [EPR3198] (Alexa Fluor® 488) - ab199213 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3198
ab199213 staining Cullin 4a in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

Anti-Cullin 4a antibody [EPR3198] (Alexa Fluor® 647) - ab199492 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3198
ab199492 staining Cullin 4a in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min)

Anti-Cullin 4a antibody - N-terminal - ab189091 from Abcam

Prices $378.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue labeling Cullin 4a with ab189091 at 1/500 dilution. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

Anti-Cullin 4A / CUL4A Antibody - LS-C332268 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
Immunohistochemistry of paraffin-embedded human cervical cancer tissue using CUL4A antibody.

Anti-Cullin 4A / CUL4A Antibody (N-Terminus) IHC-plus™ - LS-B360 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
Anti-CUL4A / Cullin 4A antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B360 dilution 1:500.

Anti-Cullin 4A / CUL4A Antibody (aa102-131) IHC-plus™ - LS-B11715 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

Anti-Cullin 4A / CUL4A Antibody (aa30-60) - LS-C167160 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
Western blot of CUL4A (arrow) using rabbit polyclonal CUL4A Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CUL4A gene.

Anti-Cullin 4A / CUL4A Antibody (aa630-679) IHC-plus™ - LS-B12021 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)

Anti-Cullin 4A / CUL4A Antibody (C-Terminus) - LS-C90299 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-Cullin 4A / CUL4A Antibody (aa625-675) - LS-C90300 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-Cullin 4A / CUL4A Antibody (aa1-20) - LS-C90301 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-Cullin 4A / CUL4A Antibody (aa30-60, AP) - LS-C221952 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-Cullin 4A / CUL4A Antibody (aa30-60, APC) - LS-C221953 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-Cullin 4A / CUL4A Antibody (aa30-60, Biotin) - LS-C221954 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-Cullin 4A / CUL4A Antibody (aa30-60, FITC) - LS-C221955 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
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