Anti-CUTL1 antibody [2A10]
| Name | Anti-CUTL1 antibody [2A10] |
|---|---|
| Supplier | Abcam |
| Catalog | ab54583 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 2A10 |
| Applications | IHC-P ICC/IF WB ICC/IF ICC/IF FC |
| Species Reactivities | Mouse, Human |
| Antigen | Recombinant fragment (proprietary-tag) corresponding to Human CUTL1 aa 521-621 |
| Description | Mouse Monoclonal |
| Gene | CUX1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemistry analysis of ab54583 antibody at 5 ug/ml with formalin-fixed, paraffin-embedded Human Spleen
Detection limit of ab54583 is approximately 0.03ng/ml as a capture antibody
![Lane 1 : Anti-CUTL1 antibody [2A10] (ab54583) (CUX1 transfected lysate)Lane 2 : Anti-CUTL1 antibody [2A10] (ab54583) (Non-transfected lysate.)Lane 1 : As aboveLane 2 : As above](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/3457_ab54583-212600-158457623050.jpg)
Lane 1 : Anti-CUTL1 antibody [2A10] (ab54583) (CUX1 transfected lysate)Lane 2 : Anti-CUTL1 antibody [2A10] (ab54583) (Non-transfected lysate.)Lane 1 : As aboveLane 2 : As above
CUTL1 antibody (ab54583) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human malignant lymphoma, diffuse large B tissue.
Western blot against tagged recombinant protein immunogen using ab54583 CUTL1 antibody at 1ug/ml.
ab54583 staining CUTL1 in human Hela cells by Immunocytochemistry / Immunofluorescence.
![Overlay histogram showing MCF7 cells stained with ab54583 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54583, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/3461_CUTL1-Primary-antibodies-ab54583-2.jpg)
Overlay histogram showing MCF7 cells stained with ab54583 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54583, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Product References
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