![Anti-CXCL5 + CXCL6 antibody [EP13083] (ab198505) at 1/5000 dilution + A549 (Human lung carcinoma) cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/3571_ab198505-240162-ab198505.jpg)
Anti-CXCL5 + CXCL6 antibody [EP13083] (ab198505) at 1/5000 dilution + A549 (Human lung carcinoma) cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
![Anti-CXCL5 + CXCL6 antibody [EP13083] (ab198505) at 1/5000 dilution + Human CLCX5 recombinant protein: aa 5-78 at 0.01 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilutiondeveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/3572_ab198505-240163-ab198505b.jpg)
Anti-CXCL5 + CXCL6 antibody [EP13083] (ab198505) at 1/5000 dilution + Human CLCX5 recombinant protein: aa 5-78 at 0.01 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilutiondeveloped using the ECL technique
![Anti-CXCL5 + CXCL6 antibody [EP13083] (ab198505) at 1/1000 dilution + Human CLCX6 recombinant protein (GST-Tag): aa 37-114 at 0.01 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilutiondeveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/3573_ab198505-240164-ab198505c.jpg)
Anti-CXCL5 + CXCL6 antibody [EP13083] (ab198505) at 1/1000 dilution + Human CLCX6 recombinant protein (GST-Tag): aa 37-114 at 0.01 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilutiondeveloped using the ECL technique
Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma tissue labeling CXCL5 + CXCL6 with ab198505 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. Inset image: negative control obtained using PBS instead of ab198505 and secondary antibody only.Note: Cytoplasm staining on human colonic carcinoma tissue was observed.
Flow cytometric analysis of A549 cells (paraformaldehyde-fixed, 2%) labeling CXCL5 + CXCL6 with ab198505 at 1/90 dilution (red) or a Rabbit monoclonal IgG (negative) (ab172730) (black), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution. Unlabeled cells (Blue).
CXCL5 + CXCL6 was immunoprecipitated from 1mg of A549 (Human lung carcinoma cell line) whole cell extract with ab198505 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab198505 at 1/1000 dilution (lanes 1 and 2). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: Input A549 whole cell extract (10µg). Lane 2: A549 whole cell extract.Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab198505 in A549 (Human lung carcinoma) whole cell extractBlocking and dilution buffer and concentration: 5% NFDM/TBST.