Anti-CXCR2 antibody [19]
| Name | Anti-CXCR2 antibody [19] |
|---|---|
| Supplier | Abcam |
| Catalog | ab24963 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 19 |
| Applications | WB B/N ELISA FC ICC/IF ICC/IF IHC-P |
| Species Reactivities | Human |
| Antigen | Synthetic peptide: MEDFNMESDS FEDFWKGED, corresponding to N terminal amino acids 1-19 of Human CXCR2 Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | CXCR2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Human peripheral blood granulocytes stained with ab24963 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab24963, 0.1μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - periphera](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/3606_ab24963-4-ab24963FC.jpg)
Human peripheral blood granulocytes stained with ab24963 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab24963, 0.1μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - periphera
Product References
Bronchial smooth muscle cells of asthmatics promote angiogenesis through elevated - Bronchial smooth muscle cells of asthmatics promote angiogenesis through elevated
Keglowich L, Roth M, Philippova M, Resink T, Tjin G, Oliver B, Lardinois D, Dessus-Babus S, Gosens R, Hostettler Haack K, Tamm M, Borger P. PLoS One. 2013 Dec 5;8(12):e81494.
Endothelial cells overexpressing interleukin-8 receptors reduce inflammatory and - Endothelial cells overexpressing interleukin-8 receptors reduce inflammatory and
Xing D, Li P, Gong K, Yang Z, Yu H, Hage FG, Oparil S, Chen YF. Circulation. 2012 Mar 27;125(12):1533-41.
Attenuation of leukocyte recruitment via CXCR1/2 inhibition stops the progression - Attenuation of leukocyte recruitment via CXCR1/2 inhibition stops the progression
Burton VJ, Holmes AM, Ciuclan LI, Robinson A, Roger JS, Jarai G, Pearce AC, Budd DC. Blood. 2011 Oct 27;118(17):4750-8.
Human IL-8 regulates smooth muscle cell VCAM-1 expression in response to - Human IL-8 regulates smooth muscle cell VCAM-1 expression in response to
Hastings NE, Feaver RE, Lee MY, Wamhoff BR, Blackman BR. Arterioscler Thromb Vasc Biol. 2009 May;29(5):725-31. doi: