All lanes : Anti-Cyclin D2 antibody (ab81359) at 1 µg/mlLane 1 : Tissue lysates prepared from Rat testicle tissue.Lane 2 : Tissue lysates prepared from Rat ovary tissue.Lane 3 : Tissue lysates prepared from Rat placental tissue.Lane 4 : Whole cell lysates prepared from MCF7 cells.Lane 5 : Whole cell lysates prepared from MM231 cells.Lane 6 : Whole cell lysates prepared from Hela cells.Lane 7 : Whole cell lysates prepared from HT1080 cells.Lane 8 : Whole cell lysates prepared from colo320 cells.Lysates/proteins at 50 µg per lane.SecondaryHRP-conjugated Goat anti-rabbit IgG at 1/3000 dilution