All lanes : Anti-Cyclin D3 antibody (ab63535) at 1/500 dilutionLane 1 : Extracts from COS7 cells (5-30ug), treated with Forskolin (40nM, 30mins), without immunising peptide.Lane 2 : Extracts from COS7 cells (5-30ug), treated with Forskolin (40nM, 30mins), with immunising peptide (5-10ug).
Ab63535 (1/50-1/100) staining human Cyclin D3 in human lung carcinoma tissue by immunohistochemistry using formalin fixed, paraffin embedded tissue. The left hand panel is without immunising peptide, whereas the right hand panel is in the presence of immunising peptide.