Anti-Cyclin D3 antibody [EP463E]

• Supplier: Abcam
• Catalog: ab52598
• Prices: $397.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP463E
• Applications: WB FC ICC/IF ICC/IF
• Species Reactivities: Mouse, Human
• Antigen: A synthetic peptide corresponding to a residue near the C-term of human Cyclin D3
• Description: Rabbit Monoclonal
• Gene: CCND3
• Conjugate: Unconjugated

Product images

Anti-Cyclin D3 antibody [EP463E] (ab52598) at 1/5000 dilution + K562 cell lysate at 10 µgSecondaryHRP-labelled goat anti-rabbit at 1/2000 dilution

Immunofluorescent staining of HeLa cells using ab52598 at a dilution of 1/100-1/250.

ab2582 staining human MDA-MB-436 breast cancer cells by Flow Cytometery. Cells were treated with Trypsin/EDTA for 5 minutes, sample buffer prepared as 5%FCS in PBS and gating was done on live non apoptotic cells. The primary antibody diluted 1/200 and incubated with sample for 1 hour at 4°C. A undiluted FITC conjugated goat polyclonal to rabbit IgG was used as secondary.See Abreview

Product References

Anticancer mechanisms of doxorubicin and zoledronic acid in breast cancer tumor - Anticancer mechanisms of doxorubicin and zoledronic acid in breast cancer tumor

Ottewell PD, Woodward JK, Lefley DV, Evans CA, Coleman RE, Holen I. Mol Cancer Ther. 2009 Oct;8(10):2821-32.