Anti-Cyclin T1 antibody
| Name | Anti-Cyclin T1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab2098 |
| Prices | $385.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IP ELISA IHC-P |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Fusion protein (Human) |
| Description | Rabbit Polyclonal |
| Gene | CCNT1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab2098 (2µg/ml) staining Cyclin T1 in human lymph node using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Anti-Cyclin T1 antibody (ab2098) at 1/10000 dilution + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
RNA elements directing in vivo assembly of the 7SK/MePCE/Larp7 transcriptional - RNA elements directing in vivo assembly of the 7SK/MePCE/Larp7 transcriptional
Muniz L, Egloff S, Kiss T. Nucleic Acids Res. 2013 Apr;41(8):4686-98.
The super elongation complex family of RNA polymerase II elongation factors: gene - The super elongation complex family of RNA polymerase II elongation factors: gene
Luo Z, Lin C, Guest E, Garrett AS, Mohaghegh N, Swanson S, Marshall S, Florens L, Washburn MP, Shilatifard A. Mol Cell Biol. 2012 Jul;32(13):2608-17.
CD4+ T cells from elite controllers resist HIV-1 infection by selective - CD4+ T cells from elite controllers resist HIV-1 infection by selective
Chen H, Li C, Huang J, Cung T, Seiss K, Beamon J, Carrington MF, Porter LC, Burke PS, Yang Y, Ryan BJ, Liu R, Weiss RH, Pereyra F, Cress WD, Brass AL, Rosenberg ES, Walker BD, Yu XG, Lichterfeld M. J Clin Invest. 2011 Apr;121(4):1549-60.
AFF4, a component of the ELL/P-TEFb elongation complex and a shared subunit of - AFF4, a component of the ELL/P-TEFb elongation complex and a shared subunit of
Lin C, Smith ER, Takahashi H, Lai KC, Martin-Brown S, Florens L, Washburn MP, Conaway JW, Conaway RC, Shilatifard A. Mol Cell. 2010 Feb 12;37(3):429-37.
Interruption of RNA processing machinery by a small compound, - Interruption of RNA processing machinery by a small compound,
Guo W, Wu S, Wang L, Wang RY, Wei X, Liu J, Fang B. Mol Cancer Ther. 2009 Feb;8(2):441-8.
Tat-SF1 is not required for Tat transactivation but does regulate the relative - Tat-SF1 is not required for Tat transactivation but does regulate the relative
Miller HB, Saunders KO, Tomaras GD, Garcia-Blanco MA. PLoS One. 2009 May 27;4(5):e5710.
Identification of the P-TEFb complex-interacting domain of Brd4 as an inhibitor - Identification of the P-TEFb complex-interacting domain of Brd4 as an inhibitor
Urano E, Kariya Y, Futahashi Y, Ichikawa R, Hamatake M, Fukazawa H, Morikawa Y, Yoshida T, Koyanagi Y, Yamamoto N, Komano J. FEBS Lett. 2008 Dec 10;582(29):4053-8.
Peroxisome proliferator-activated receptor gamma recruits the positive - Peroxisome proliferator-activated receptor gamma recruits the positive
Iankova I, Petersen RK, Annicotte JS, Chavey C, Hansen JB, Kratchmarova I, Sarruf D, Benkirane M, Kristiansen K, Fajas L. Mol Endocrinol. 2006 Jul;20(7):1494-505. Epub 2006 Feb 16.
Regulation of polymerase II transcription by 7SK snRNA: two distinct RNA elements - Regulation of polymerase II transcription by 7SK snRNA: two distinct RNA elements
Egloff S, Van Herreweghe E, Kiss T. Mol Cell Biol. 2006 Jan;26(2):630-42.