Anti-Cyclophilin 40 antibody [EPR14844] - N-terminal (ab186727) at 1/1000 dilution + JAR cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-Cyclophilin 40 antibody [EPR14844] - N-terminal (ab186727) at 1/10000 dilutionLane 1 : K562 cell lysateLane 2 : HepG2 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Western blot analysis of Cyclophilin 40 in immunoprecipitation pellets from K562 lysate. ab186727 used at 1/50 dilution (Lane 1) or PBS (Lane 2). Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a1/1500 dilution.