Anti-Cyclophilin A antibody
| Name | Anti-Cyclophilin A antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab41684 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human, Bovine, Dog, Pig, Monkey, Ape, Hamster, Orangutan |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human Cyclophilin A |
| Description | Rabbit Polyclonal |
| Gene | PPIA |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Cyclophilin A antibody (ab41684) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell LysateLane 3 : Lymph node (Human) Tissue Lysate - adult normal tissue (ab29871)Lane 4 : Thymus (Human) Tissue Lysate - adult normal tissue (ab30146)Lane 5 : Lung (Rat) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
ICC/IF image of ab41684 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab41684, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
ab41684 staining mouse heart and rat thymus tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking in sequential peroxidase and protein block (prediluted) for 20 minutes at 20°C. The primay antibody was diluted 1/100 and incubated with the samples for 45 minutes at 20°C. A HRP-conjugated goat anti-rabbit anitbody was used as the secondary.See Abreview
Anti-Cyclophilin A antibody (ab41684) at 1 µg/ml + Cyclophilin A protein (Human) (ab88202) at 0.01 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 30 seconds
Product References
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