PPIA

Gene Symbol	PPIA
Entrez Gene	5478
Alt Symbol	CYPA, CYPH, HEL-S-69p
Species	Human
Gene Type	protein-coding
Description	peptidylprolyl isomerase A (cyclophilin A)
Other Description	PPIase A\|T cell cyclophilin\|cyclosporin A-binding protein\|epididymis secretory sperm binding protein Li 69p\|peptidyl-prolyl cis-trans isomerase A\|rotamase A
Swissprots	Q9BRU4 Q3KQW3 Q96IX3 P05092 A8K220 Q9BTY9 Q567Q0 Q6IBU5 Q9UC61 P62937
Accessions	AAU13906 CAA37039 P62937 AB451307 BAG70121 AB451438 BAG70252 AK026569 AK130101 AK290085 BAF82774 AK290851 BAF83540 AK293003 BAF85692 AK297340 BAG59794 AK316564 BAG38153 AU100120 BC000689 AAH00689 BC003026 AAH03026 BC005320 AAH05320 BC005982 AAH05982 BC007104 AAH07104 BC013915 AAH13915 BC018843 BC037419 BC073992 AAH73992 BC093076 AAH93076 BC106030 AAI06031 BC137057 AAI37058 BC137058 AAI37059 BM806224 BM909285 BU786475 BX347265 BX408703 CD014106 CR456707 CAG32988 D28351 BAA05717 DQ892625 ABM83551 DQ895865 ABM86791 EU794650 ACJ13704 Y00052 CAA68264 NM_001300981 NP_001287910 NM_021130 NP_066953 NM_203430 NM_203431
Function	PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Subcellular Location	Cytoplasm {ECO:0000269\|PubMed:16527992}. Secreted {ECO:0000269\|PubMed:16527992}. Note=Secretion occurs in response to oxidative stress in vascular smooth muscle through a vesicular secretory pathway that involves actin remodeling and myosin II activation, and mediates ERK1/2 activation.

Cyclophilin A Antibody - 2175 from Cell Signaling Technology

Prices	$246.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of extracts from HeLa (human), NIH/3T3 (mouse) and KNRK (rat) cell lines, using Cyclophilin A Antibody.

CyPA (H-24) - sc-133494 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	100 µg/ml
Host	Rabbit
Clonality	Polyclonal

CyPA (H-24): sc-133494. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human intestine tissue showing cytoplasmic localization.

CyPA (6-YD13) - sc-134310 from Santa Cruz Biotechnology

Prices	$295.00
Sizes	100 µg/ml
Host	Mouse
Clonality	Monoclonal

CyPA (6-YD13): sc-134310. Western blot analysis of CyPA expression in K-562 whole cell lysate.

Anti-Cyclophilin A antibody - ab41684 from Abcam

Prices	$401.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-Cyclophilin A antibody (ab41684) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell LysateLane 3 : Lymph node (Human) Tissue Lysate - adult normal tissue (ab29871)Lane 4 : Thymus (Human) Tissue Lysate - adult normal tissue (ab30146)Lane 5 : Lung (Rat) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

Anti-Cyclophilin A antibody - ab3563 from Abcam

Prices	$400.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

ab3563 at 1/300 dilution detecting 0.5ug recombinant human cyclophilin A by Western Blot (ECL).

Anti-Cyclophilin A antibody - ab58144 from Abcam

Prices	$403.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal

Anti-Cyclophilin A antibody - ab42408 from Abcam

Prices	$394.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-Cyclophilin A antibody (ab42408) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell LysateLane 3 : Lymph node (Human) Tissue Lysate - adult normal tissue (ab29871)Lane 4 : Thymus (Human) Tissue Lysate - adult normal tissue (ab30146)Lane 5 : Lung (Rat) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

Anti-Cyclophilin A antibody - ab137684 from Abcam

Prices	$384.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-Cyclophilin A antibody (ab137684) at 1/5000 dilutionLane 1 : H1299 whole cell lysateLane 2 : HeLa S3 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-Cyclophilin A antibody - ab154388 from Abcam

Prices	$376.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-Cyclophilin A antibody (ab154388) at 1/1000 dilutionLane 1 : Molt-4 whole cell lysateLane 2 : Raji whole cell lysateLysates/proteins at 30 µg per lane.

Anti-Cyclophilin A antibody - ab77188 from Abcam

Prices	$376.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

Anti-Cyclophilin A antibody (ab77188) at 0.1 µg/ml + Human Lymph node lysate (RIPA buffer) at 35 µg

Anti-Cyclophilin A antibody [EPR7510] - ab131334 from Abcam

Prices	$379.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR7510

All lanes : Anti-Cyclophilin A antibody [EPR7510] (ab131334) at 1/10000 dilutionLane 1 : K562 cell lysateLane 2 : Jurkat cell lysateLane 3 : HepG2 cell lysateLane 4 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Anti-Cyclophilin A antibody [EPR7511] - ab126738 from Abcam

Prices	$385.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR7511

All lanes : Anti-Cyclophilin A antibody [EPR7511] (ab126738) at 1/1000 dilutionLane 1 : K562 lysateLane 2 : HeLa LysateLane 3 : Jurkat lysateLane 4 : HepG2 lysateLysates/proteins at 10 µg per lane.SecondaryGoat-anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL technique

Anti-Cyclophilin A antibody [ZC001] - ab54218 from Abcam

Prices	$376.00
Sizes	500 µl
Host	Mouse
Clonality	Monoclonal
Clone	ZC001

Anti-Cyclophilin A antibody [ZC001] (ab54218) at 0.3 µg/ml + T47D cell lysate

Anti-PPIA / Cyclophilin A Antibody - LS-C22578 from LifeSpan Bioscience

Prices	$355.00
Sizes	50 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PPIA / Cyclophilin A Antibody - LS-C22579 from LifeSpan Bioscience

Prices	$665.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PPIA / Cyclophilin A Antibody - LS-C22580 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PPIA / Cyclophilin A Antibody (C-Terminus) - LS-C36063 from LifeSpan Bioscience

Prices	$405.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PPIA / Cyclophilin A Antibody (Internal) - LS-C145031 from LifeSpan Bioscience

Prices	$365.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PPIA / Cyclophilin A Antibody (Internal) - LS-C153408 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-PPIA / Cyclophilin A Antibody (aa1-166, clone 10B2627, Azide-free) - LS-C183545 from LifeSpan Bioscience

Prices	$560.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	10B2627

Anti-PPIA / Cyclophilin A Antibody (aa1-185) - LS-C189799 from LifeSpan Bioscience

Prices	$650.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

Anti-PPIA / Cyclophilin A Antibody (Internal, clone EPR7510) - LS-C154563 from LifeSpan Bioscience

Prices	$475.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR7510

Western blot analysis on (A) K562, (B) Jurkat, (C) HepG2, and (D) HeLa cell lysates using anti-Cyclophilin A antibody.

Anti-PPIA / Cyclophilin A Antibody (aa36-85) - LS-C81639 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Fetal brain lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-PPIA / Cyclophilin A Antibody (aa83-95) - LS-C54690 from LifeSpan Bioscience

Prices	$395.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

Antibody (2 ug/ml) staining of human lymph node lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-PPIA / Cyclophilin A Antibody (Biotin) - LS-C212163 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western blot of Peptidyl-prolyl cis-trans isomerase A antibody at 2 ug/ml. Lane 1:293T whole cell lysate. Lane 2: EC109 whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 18 kDa. Observed band size: 18 kDa Additional bands at: 80 kDa. We are unsure as to the identity of this extra band.

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