Anti-Cyclophilin A antibody
| Name | Anti-Cyclophilin A antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab3563 |
| Prices | $400.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB ELISA ICC/IF |
| Species Reactivities | Mouse, Rat, Rabbit, Hamster, Human, Bovine, Cat, Pig, Chimpanzee, Monkey, Monkey |
| Antigen | Other Immunogen Type corresponding to Human Cyclophilin A |
| Description | Rabbit Polyclonal |
| Gene | PPIA |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab3563 at 1/300 dilution detecting 0.5ug recombinant human cyclophilin A by Western Blot (ECL).
ICC/IF image of ab3563 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3563, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-Cyclophilin A antibody (ab3563) at 1/500 dilutionLane 1 : HeLa cell lysateLane 2 : K562 cell lysateLane 3 : HepG2 cell lysateLane 4 : NIH-3T3Lysates/proteins at 25 µg per lane.Observed band size : 18 kDa (why is the actual band size different from the predicted?)
Product References
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Fan YN, Meley D, Pizer B, See V. PLoS One. 2014 Sep 24;9(9):e108514.
A major prolactin-binding complex on human milk fat globule membranes contains - A major prolactin-binding complex on human milk fat globule membranes contains
Lorenson MY, Ueda EK, Chen KE, Walker AM. Am J Physiol Endocrinol Metab. 2012 Mar 1;302(5):E585-94. doi:
The use of AlphaLISA technology to detect interaction between hepatitis C - The use of AlphaLISA technology to detect interaction between hepatitis C
Waller H, Chatterji U, Gallay P, Parkinson T, Targett-Adams P. J Virol Methods. 2010 May;165(2):202-10.
Stress responses and conditioning effects in mesothelial cells exposed to - Stress responses and conditioning effects in mesothelial cells exposed to
Kratochwill K, Lechner M, Siehs C, Lederhuber HC, Rehulka P, Endemann M, Kasper DC, Herkner KR, Mayer B, Rizzi A, Aufricht C. J Proteome Res. 2009 Apr;8(4):1731-47.
Dynamic proteomics of individual cancer cells in response to a drug. - Dynamic proteomics of individual cancer cells in response to a drug.
Cohen AA, Geva-Zatorsky N, Eden E, Frenkel-Morgenstern M, Issaeva I, Sigal A, Milo R, Cohen-Saidon C, Liron Y, Kam Z, Cohen L, Danon T, Perzov N, Alon U. Science. 2008 Dec 5;322(5907):1511-6.