Anti-Cyclophilin B antibody
| Name | Anti-Cyclophilin B antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab16045 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P ICC/IF ICC/IF IHC-F IP |
| Species Reactivities | Mouse, Rat, Horse, Chicken, Dog, Human, Bovine, Pig, Xenopus |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human Cyclophilin B |
| Description | Rabbit Polyclonal |
| Gene | PPIB |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab16045 stained HeLa cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16045 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
All lanes : Anti-Cyclophilin B antibody (ab16045) at 1 µg/mlLane 1 : Rat LiverLane 2 : Mouse 3T3Lane 3 : DogLane 4 : ChickenLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilutionPerformed under reducing conditions.
Performed under reducing conditions.
Cyclophilin B was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Cyclophilin B and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab16045.Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.Band: 21kDa: Cyclophilin B.
ICC/IF image of ab16045 stained NIH/3T3 cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab16045, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
ab16045 (1/1000) staining Cyclophilin B in assynchronous HeLa cells (green). Cells were fixed with Paraformaldehyde and counter-stained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.See Abreview
Image courtesy of Human Protein Atlas ab16045 staining in human placenta, showing staining of the cytrotrophoblasts. Paraffin embedded placental tissue was incubated with ab16045 (1:14,000 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab16045 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org.
All lanes : Anti-Cyclophilin B antibody (ab16045) at 1/1000 dilutionLane 1 : Whole cell lysate prepared from rat pancreatic AR42J cells, which were treated with 10nM dexamethasone for 48 hours.Lane 2 : Whole cell lysate for negative control, prepared from rat pancreatic AR42J cells (specific knock down of cyclophilin B/PpiB by siRNA), which were treated with 10nM dexamethasone for 48 hours.SecondaryGoat-anti-Rabbit HRP-conjugated polyclonal at 1/2000 dilutiondeveloped using the ECL technique
developed using the ECL techniquePerformed under reducing conditions.
Product References
Tubular p53 regulates multiple genes to mediate AKI. - Tubular p53 regulates multiple genes to mediate AKI.
Zhang D, Liu Y, Wei Q, Huo Y, Liu K, Liu F, Dong Z. J Am Soc Nephrol. 2014 Oct;25(10):2278-89.
Depletion of cyclophilins B and C leads to dysregulation of endoplasmic reticulum - Depletion of cyclophilins B and C leads to dysregulation of endoplasmic reticulum
Stocki P, Chapman DC, Beach LA, Williams DB. J Biol Chem. 2014 Aug 15;289(33):23086-96.
Cyclophilin A associates with enterovirus-71 virus capsid and plays an essential - Cyclophilin A associates with enterovirus-71 virus capsid and plays an essential
Qing J, Wang Y, Sun Y, Huang J, Yan W, Wang J, Su D, Ni C, Li J, Rao Z, Liu L, Lou Z. PLoS Pathog. 2014 Oct 2;10(10):e1004422.
Amyloid-beta oligomers induce differential gene expression in adult human brain - Amyloid-beta oligomers induce differential gene expression in adult human brain
Sebollela A, Freitas-Correa L, Oliveira FF, Paula-Lima AC, Saraiva LM, Martins SM, Mota LD, Torres C, Alves-Leon S, de Souza JM, Carraro DM, Brentani H, De Felice FG, Ferreira ST. J Biol Chem. 2012 Mar 2;287(10):7436-45.
Proteomic assessment shows that many endoplasmic reticulum (ER)-resident proteins - Proteomic assessment shows that many endoplasmic reticulum (ER)-resident proteins
Pehar M, Lehnus M, Karst A, Puglielli L. J Biol Chem. 2012 Jun 29;287(27):22436-40.
Cell-type-dependent regulation of mTORC1 by REDD1 and the tumor suppressors - Cell-type-dependent regulation of mTORC1 by REDD1 and the tumor suppressors
Wolff NC, Vega-Rubin-de-Celis S, Xie XJ, Castrillon DH, Kabbani W, Brugarolas J. Mol Cell Biol. 2011 May;31(9):1870-84.
Mutations in PPIB (cyclophilin B) delay type I procollagen chain association and - Mutations in PPIB (cyclophilin B) delay type I procollagen chain association and
Pyott SM, Schwarze U, Christiansen HE, Pepin MG, Leistritz DF, Dineen R, Harris C, Burton BK, Angle B, Kim K, Sussman MD, Weis M, Eyre DR, Russell DW, McCarthy KJ, Steiner RD, Byers PH. Hum Mol Genet. 2011 Apr 15;20(8):1595-609.
Novel embryonic neuronal migration and proliferation defects in Dcx mutant mice - Novel embryonic neuronal migration and proliferation defects in Dcx mutant mice
Pramparo T, Youn YH, Yingling J, Hirotsune S, Wynshaw-Boris A. J Neurosci. 2010 Feb 24;30(8):3002-12.
Stoichiometry of expressed alpha(4)beta(2)delta gamma-aminobutyric acid type A - Stoichiometry of expressed alpha(4)beta(2)delta gamma-aminobutyric acid type A
Wagoner KR, Czajkowski C. J Biol Chem. 2010 May 7;285(19):14187-94.
Analysis of low abundance membrane-associated proteins from rat pancreatic - Analysis of low abundance membrane-associated proteins from rat pancreatic
Borta H, Aroso M, Rinn C, Gomez-Lazaro M, Vitorino R, Zeuschner D, Grabenbauer M, Amado F, Schrader M. J Proteome Res. 2010 Oct 1;9(10):4927-39.