PPIB

Gene Symbol PPIB
Entrez Gene 5479
Alt Symbol CYP-S1, CYPB, HEL-S-39, OI9, SCYLP
Species Human
Gene Type protein-coding
Description peptidylprolyl isomerase B (cyclophilin B)
Other Description PPIase B|S-cyclophilin|cyclophilin-like protein|epididymis secretory protein Li 39|peptidyl-prolyl cis-trans isomerase B|rotamase B
Swissprots A8K534 Q9BVK5 Q6IBH5 P23284
Accessions AAX44050 CAJ44951 CBF57604 EAW77669 P23284 AK291149 BAF83838 AK311074 BC001125 AAH01125 BC008848 AAH08848 BC020800 AAH20800 BC032138 AAH32138 BQ931949 CR456829 CAG33110 D28465 BAA05831 DQ892289 ABM83215 DQ895488 ABM86414 EU794651 ACJ13705 M60457 AAA35733 M60857 AAA52150 M63573 AAA36601 U95646 NM_000942 NP_000933
Function PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Subcellular Location Endoplasmic reticulum lumen. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

CyP (E-8) - sc-390193 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CyP (E-8): sc-390193. Western blot analysis of CyP expression in A-431 (A) and HeLa (B) whole cell lysates.

CyPB (C-15) - sc-20361 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CyPB (C-15): sc-20361. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

CyPB (G-13) - sc-31125 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CyPB (G-13): sc-31125. Western blot analysis of CyPB expression in THP-1 (A), U-87 MG (B), Hep G2 (C), HeLa (D), MCF7 (E) and A2058 (F) whole cell lysates.

CyPB (T-15) - sc-31124 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

CyPB (k2E2) - sc-130626 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
CyPB (k2E2): sc:130626. Western blot analysis of CyPB expression in Hep G2 (A) and HeLa (B) whole cell lysates.

Anti-Cyclophilin B antibody - ab16045 from Abcam

Prices $401.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab16045 stained HeLa cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16045 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-Cyclophilin B antibody - ab171122 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lanes 1 & 3 : Anti-Cyclophilin B antibody (ab171122) at 1/2000 dilutionLane 2 : Anti-Cyclophilin B antibody (ab171122) at 1/1000 dilutionLane 4 : Anti-Cyclophilin B antibody (ab171122) at 1/4000 dilutionLane 1 : rat liver lysateLane 2 : rat liver lysateLane 3 : rat liver lysateLane 4 : rat liver lysateLysates/proteins at 25 µg per lane.

Anti-Cyclophilin B antibody - ab154371 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Cyclophilin B antibody (ab154371) at 1/500 dilutionLane 1 : A431 whole cell lysateLane 2 : HepG2 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-Cyclophilin B antibody - ab115658 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-Cyclophilin B antibody [EPR12703(B)] - Loading Control - ab178397 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR12703(B)
All lanes : Anti-Cyclophilin B antibody [EPR12703(B)] - Loading Control (ab178397) at 1/1000 dilutionLane 1 : HepG2 cell lysateLane 2 : HeLa cell lysateLane 3 : A431 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL technique

Anti-Cyclophilin B antibody [k2E2] - ab74173 from Abcam

Prices $387.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone k2E2
All lanes : Anti-Cyclophilin B antibody [k2E2] (ab74173) at 1/1000 dilutionLane 1 : HepG2 cell lysateLane 2 : HeLa cell lysateLysates/proteins at 30 µg per lane.Secondarygoat anti-mouse antibody conjugated to HRP developed using the ECL technique

Anti-PPIB / Cyclophilin B Antibody (aa34-216) - LS-C124938 from LifeSpan Bioscience

Prices $465.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-PPIB / Cyclophilin B Antibody (aa34-216) - LS-C293602 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PPIB / Cyclophilin B Antibody (aa34-216) - LS-C293603 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PPIB / Cyclophilin B Antibody (aa25-207, Biotin) - LS-C297902 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PPIB / Cyclophilin B Antibody (aa34-216, Biotin) - LS-C297903 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PPIB / Cyclophilin B Antibody (aa47-216, Biotin) - LS-C297904 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PPIB / Cyclophilin B Antibody (aa34-216, Biotin) - LS-C297905 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PPIB / Cyclophilin B Antibody (aa25-207, FITC) - LS-C301562 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PPIB / Cyclophilin B Antibody (aa47-216, FITC) - LS-C301563 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PPIB / Cyclophilin B Antibody (aa34-216, FITC) - LS-C301564 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PPIB / Cyclophilin B Antibody (aa34-216, FITC) - LS-C301567 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PPIB / Cyclophilin B Antibody (aa100-200) - LS-C148529 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of Cyclophilin B in A) HeLa cell lysate and B) 293 cell lysate using LS-C148529 at 0.5 and 2 ug/ml, respectively. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution, were used for this test.

Anti-PPIB / Cyclophilin B Antibody (aa25-207) - LS-C293599 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of recombinant PPIB / Cyclophilin B.

Anti-PPIB / Cyclophilin B Antibody (aa26-216, clone K2E2) - LS-C93715 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone K2E2
Cell lysates of HepG2(30 ug) and HeLa(30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Cyclophilin B (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection.
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