Anti-CYP1B1 antibody
| Name | Anti-CYP1B1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab32649 |
| Prices | $395.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-P |
| Species Reactivities | Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human CYP1B1 |
| Description | Rabbit Polyclonal |
| Gene | CYP1B1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-CYP1B1 antibody (ab32649) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissueLane 2 : Kidney (Human) Tissue Lysate - adult normal tissueLane 3 : Liver (Human) Tissue Lysate - adult normal tissueLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Image courtesy of Human Protein Atlasab32649 staining CYP1B1 in human stomach. Paraffin embedded human stomach tissue was incubated with ab32649 (1/1500 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.ab32649 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org.
ICC/IF image of ab32649 stained HeLa cells. The cells were 4% formaldehyde fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab32649 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Disturbed balance between phase I and II metabolizing enzymes in ovarian - Disturbed balance between phase I and II metabolizing enzymes in ovarian
Hevir N, Ribic-Pucelj M, Lanisnik Rizner T. Mol Cell Endocrinol. 2013 Mar 10;367(1-2):74-84.
Disturbed expression of phase I and phase II estrogen-metabolizing enzymes in - Disturbed expression of phase I and phase II estrogen-metabolizing enzymes in
Hevir N, Sinkovec J, Rizner TL. Mol Cell Endocrinol. 2011 Jan 1;331(1):158-67.