All lanes : Anti-CYP1B1 antibody [EPR14972] - C-terminal (ab185954) at 1/20000 dilutionLane 1 : Human breast cancer lysateLane 2 : Human fetal kidney lysateLysates/proteins at 20 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human renal adenocarcinoma tissue labeling CYP1B1 with ab185954 at 1/500 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling CYP1B1 with ab185954 at 1/200 dilution (red) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.
All lanes : Anti-CYP1B1 antibody [EPR14972] - C-terminal (ab185954) at 1/5000 dilutionLane 1 : Rat brain lysateLane 2 : Rat spleen lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-CYP1B1 antibody [EPR14972] - C-terminal (ab185954) at 1/5000 dilution + Human fetal brain lysate at 20 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution