Anti-CYP27A1 antibody
| Name | Anti-CYP27A1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab64889 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ICC/IF ICC/IF ELISA WB |
| Species Reactivities | Human |
| Antigen | Synthetic peptide (Human) from an internal sequence |
| Description | Rabbit Polyclonal |
| Gene | CYP27A1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-CYP27A1 antibody (ab64889) at 1/500 dilutionLane 1 : extracts from HeLa cells (5-30µg total protein) Lane 2 : extracts from HeLa cells (5-30µg total protein) and 5-10µg of the immunising peptide.
ab64889 staining CYP27A1 in human colon tissue sections by IHC-P (formaldehyde-fixed paraffin-embedded sections). Tissue samples were fixed with formaldehyde and blocked with 0.4% Casein in phosphate-buffered saline for 30 minutes at 25°C. Antigen retrieval was by heat mediation in target retrieval solution. Samples were incubated with primary antibody 1/2000 (TBST) for 1 hour at 25°C. See Abreview
ICC/IF image of ab64889 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64889, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
De novo synthesis of steroids and oxysterols in adipocytes. - De novo synthesis of steroids and oxysterols in adipocytes.
Li J, Daly E, Campioli E, Wabitsch M, Papadopoulos V. J Biol Chem. 2014 Jan 10;289(2):747-64.
Role of local bioactivation of vitamin D by CYP27A1 and CYP2R1 in the control of - Role of local bioactivation of vitamin D by CYP27A1 and CYP2R1 in the control of
Bergada L, Pallares J, Maria Vittoria A, Cardus A, Santacana M, Valls J, Cao G, Fernandez E, Dolcet X, Dusso AS, Matias-Guiu X. Lab Invest. 2014 Jun;94(6):608-22.
A functional assay for microRNA target identification and validation. - A functional assay for microRNA target identification and validation.
Gaken J, Mohamedali AM, Jiang J, Malik F, Stangl D, Smith AE, Chronis C, Kulasekararaj AG, Thomas NS, Farzaneh F, Tavassoli M, Mufti GJ. Nucleic Acids Res. 2012 May;40(10):e75.