Anti-Cystathionase antibody
| Name | Anti-Cystathionase antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab54573 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | WB IHC-P ICC/IF ICC/IF FC |
| Species Reactivities | Human |
| Antigen | Recombinant full length protein, corresponding to amino acids 1-406 of Human Cystathionase |
| Description | Mouse Monoclonal |
| Gene | CTH |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Cystathionase antibody (ab54573) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human colon.
ICC/IF image of ab54573 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54573, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Overlay histogram showing K562 cells stained with ab54573 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54573, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in K562 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/4551_Cystathionase-Primary-antibodies-ab54573-2.jpg)
Overlay histogram showing K562 cells stained with ab54573 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54573, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in K562 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
Effect of feeding a high fat diet on hydrogen sulfide (H2S) metabolism in the - Effect of feeding a high fat diet on hydrogen sulfide (H2S) metabolism in the
Peh MT, Anwar AB, Ng DS, Atan MS, Kumar SD, Moore PK. Nitric Oxide. 2014 Sep 15;41:138-45.
H2S-Induced sulfhydration of the phosphatase PTP1B and its role in the - H2S-Induced sulfhydration of the phosphatase PTP1B and its role in the
Krishnan N, Fu C, Pappin DJ, Tonks NK. Sci Signal. 2011 Dec 13;4(203):ra86.
Astrocytes produce the antiinflammatory and neuroprotective agent hydrogen - Astrocytes produce the antiinflammatory and neuroprotective agent hydrogen
Lee M, Schwab C, Yu S, McGeer E, McGeer PL. Neurobiol Aging. 2009 Oct;30(10):1523-34. doi: