All lanes : Anti-Cystatin C antibody [1A6] (ab128082) at 1/2000 dilutionLane 1 : lysate from HEK293T cells transfected with the pCMV6-ENTRY control vector for 48 hrsLane 2 : lysate from HEK293T cells transfected with the pCMV6-ENTRY Cystatin C vector for 48 hrsLysates/proteins at 5 µg per lane.
All lanes : Anti-Cystatin C antibody [1A6] (ab128082) at 1/2000 dilutionLane 1 : HepG2 cell extractsLane 2 : HeLa cell extractsLane 3 : SVT2 cell extractsLane 4 : A549 cell extractsLane 5 : COS7 cell extractsLane 6 : Jurkat cell extractsLane 7 : MDCK cell extractsLane 8 : PC12 cell extractsLane 9 : MCF7 cell extractsLysates/proteins at 35 µg per lane.
ab128082, at 1/100 dilution, staining Cystatin C in HeLa cells, by Immunofluorescence.