CST3

Gene Symbol	CST3
Entrez Gene	1471
Alt Symbol	ARMD11
Species	Human
Gene Type	protein-coding
Description	cystatin C
Other Description	bA218C14.4 (cystatin C)\|cystatin 3\|cystatin-3\|cystatin-C\|gamma-trace\|neuroendocrine basic polypeptide\|post-gamma-globulin
Swissprots	P01034 B2R5J9 D3DW42 Q6FGW9
Accessions	AAA52164 CAA36497 CAA43856 CAJ44947 CBF57600 EAX10137 EAX10138 P01034 AB587083 BAJ83816 AK312213 BAG35146 BC013083 AAH13083 BC110305 AAI10306 BF509512 BT006839 AAP35485 BX647523 CR541988 CAG46785 CR542018 CAG46815 DQ891439 ABM82365 EU176584 ABW03385 HY042866 X05607 CAA29096 NM_000099 NP_000090 NM_001288614 NP_001275543
Function	As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
Subcellular Location	Secreted {ECO:0000269\|PubMed:20189825}.
Tissue Specificity	Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland. {ECO:0000269\|PubMed:15274116, ECO:0000269\|PubMed:20189825}.
Top Pathways	Salivary secretion

cystatin C (P-14) - sc-16989 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

cystatin C (P-14): sc-16989. Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in tubules.

cystatin C (Cyst-18) - sc-66100 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	100 µg/ml
Host	Mouse
Clonality	Monoclonal

cystatin C (C-27) - sc-73878 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	100 µg/ml
Host	Mouse
Clonality	Monoclonal

cystatin C (C-27): sc-73878. Western blot analysis of cystatin C expression in non-transfected: sc-110760 (A) and human cystatin C transfected: sc-112728 (B) 293 whole cell lysates.

cystatin C (Cyst-13) - sc-51857 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	100 µg/ml
Host	Mouse
Clonality	Monoclonal

Anti-Cystatin C antibody - ab68290 from Abcam

Prices	$384.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Cystatin C was immunoprecipitated using 0.5mg Caco2 whole cell extract, 5µg of Rabbit polyclonal to Cystatin C and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Caco2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab68290.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 14kDa; Cystatin C

Anti-Cystatin C antibody - ab85790 from Abcam

Prices	$384.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Standard Curve for Cystatin C (Analyte: ab7653) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [Cyst-13] to Cystatin C (ab24327) at 1ug/ml and Detector Antibody Rabbit polyclonal to Cystatin C (ab85790) at 0.5ug/ml

Anti-Cystatin C antibody - ab117642 from Abcam

Prices	$376.00
Sizes	50 µg
Host	Goat
Clonality	Polyclonal

Human Kidney, Tubules, Formalin-Fixed, Paraffin-Embedded tissue, stained with ab117642 at 5µg/ml.

Anti-Cystatin C antibody - ab85345 from Abcam

Prices	$376.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

Anti-Cystatin C antibody (ab85345) at 1 µg/ml + Human Testis lysate at 35 µg

Anti-Cystatin C antibody - ab115872 from Abcam

Prices	$370.00
Sizes	1 mg
Host	Goat
Clonality	Polyclonal

Anti-Cystatin C antibody [1A6] - ab128082 from Abcam

Prices	$376.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	1A6

All lanes : Anti-Cystatin C antibody [1A6] (ab128082) at 1/2000 dilutionLane 1 : lysate from HEK293T cells transfected with the pCMV6-ENTRY control vector for 48 hrsLane 2 : lysate from HEK293T cells transfected with the pCMV6-ENTRY Cystatin C vector for 48 hrsLysates/proteins at 5 µg per lane.

Anti-Cystatin C antibody [Cyst-13] - ab24327 from Abcam

Prices	$390.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	Cyst-13

Anti-Cystatin C antibody [EPR4413] - ab109508 from Abcam

Prices	$381.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR4413

All lanes : Anti-Cystatin C antibody [EPR4413] (ab109508) at 1/10000 dilutionLane 1 : Human testis lysateLane 2 : HeLa testis lysateLane 3 : HepG2 testis lysateLane 4 : U87-MG testis lysateLysates/proteins at 10 µg per lane.

Anti-Cystatin C antibody [EPR4502] - ab133495 from Abcam

Prices	$379.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR4502

All lanes : Anti-Cystatin C antibody [EPR4502] (ab133495) at 1/1000 dilutionLane 1 : Human testis lysateLane 2 : Human brain lysateLane 3 : PC3 lysateLane 4 : HepG2 lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-Cystatin C antibody [EPSISR32] - ab168357 from Abcam

Prices	$379.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPSISR32

All lanes : Anti-Cystatin C antibody [EPSISR32] (ab168357) at 1/1000 dilutionLane 1 : Human fetal brain lysateLane 2 : Human testis lysateLane 3 : HepG2 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labeled goat anti-rabbit at 1/2000 dilution

Anti-Cystatin C antibody [MM0229-3N39] - ab97730 from Abcam

Prices	$370.00
Sizes	50 µg
Host	Mouse
Clonality	Monoclonal
Clone	MM0229-3N39

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placental tissue section (4µm) labelling CD239 Cystatin C ab97730. Tissue was treated with 15µg/ml Protenase K for antigen retrieval and fixed with 4% PFA. A HRP-conjugated anti-mouse IgG was used as the secondary antibody (30 minutes at room temperature) followed by DAB color development.

Anti-CST3 / Cystatin C Antibody (aa27-146, FITC) - LS-C305381 from LifeSpan Bioscience

Host	Rabbit
Clonality	Polyclonal

Anti-CST3 / Cystatin C Antibody (aa32-148) - LS-C293618 from LifeSpan Bioscience

Host	Rabbit
Clonality	Polyclonal

Western blot of recombinant CST3 / Cystatin C.

Anti-CST3 / Cystatin C Antibody (aa46-147) - LS-C293615 from LifeSpan Bioscience

Host	Rabbit
Clonality	Polyclonal

Anti-CST3 / Cystatin C Antibody (aa27-146) - LS-C293616 from LifeSpan Bioscience

Host	Rabbit
Clonality	Polyclonal

Anti-CST3 / Cystatin C Antibody (clone 5H2) - LS-C169351 from LifeSpan Bioscience

Host	Mouse
Clonality	Monoclonal
Clone	5H2

Western blot using CST3 mouse monoclonal antibody against HeLa (1) and Caco-2 (2) cell lysate.

Anti-CST3 / Cystatin C Antibody (aa21-140, FITC) - LS-C305382 from LifeSpan Bioscience

Host	Rabbit
Clonality	Polyclonal

Anti-CST3 / Cystatin C Antibody IHC-plus™ - LS-B10633 from LifeSpan Bioscience

Host	Rabbit
Clonality	Polyclonal

Anti-CST3 / Cystatin C antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10633 concentration 10 ug/ml.

Anti-CST3 / Cystatin C Antibody - LS-C315771 from LifeSpan Bioscience

Host	Goat
Clonality	Polyclonal

Anti-CST3 / Cystatin C Antibody - LS-C315772 from LifeSpan Bioscience

Host	Goat
Clonality	Polyclonal

Anti-CST3 / Cystatin C Antibody (FITC) - LS-C317193 from LifeSpan Bioscience

Host	Rabbit
Clonality	Polyclonal

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