Anti-Cystatin C antibody [Cyst-13]
| Name | Anti-Cystatin C antibody [Cyst-13] |
|---|---|
| Supplier | Abcam |
| Catalog | ab24327 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | Cyst-13 |
| Applications | ELISA ELISA ICC/IF ICC/IF WB |
| Species Reactivities | Human |
| Antigen | Full length Cystatin C protein (Human) conjugated to BSA |
| Description | Mouse Monoclonal |
| Gene | CST3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Standard Curve for Cystatin C (Analyte: ab7653) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [Cyst-13] to Cystatin C (ab24327) at 1ug/ml and Detector Antibody Rabbit polyclonal to Cystatin C (ab85790) at 0.5ug/ml](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/4583_Cystatin-C-Primary-antibodies-ab24327-5.jpg)
Standard Curve for Cystatin C (Analyte: ab7653) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [Cyst-13] to Cystatin C (ab24327) at 1ug/ml and Detector Antibody Rabbit polyclonal to Cystatin C (ab85790) at 0.5ug/ml
ICC/IF image of ab24327 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24327, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.