Anti-Cytochrome b reductase 1 antibody (ab66048) at 1 µg/ml + A20 (Mouse B lymphoma cell line) Whole Cell Lysate (ab7180) at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
IHC-Fr image of human duodenum section stained with ab66048. The sections were incubated in 10% normal donkey serum in 0.1% PBS- and 0.3x triton100 for 1h to permeabilise the cells and to block non-specific protein-protein interactions. The sections were then incubated with the antibody ab66048, 1µg/ml) and Anti-Actin overnight at +4°C. The secondary antibody was Alexa Alexa Fluor® 488 goat anti-rabbit IgG (H+L) and Fluor®568 donkey anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. dCytb stained apical site of duodenum and actin stained muscles and blood vessels.See Abreview
ICC/IF image of ab66048 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66048, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.