Anti-Cytochrome b245 Light Chain antibody
| Name | Anti-Cytochrome b245 Light Chain antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab75941 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human, Bovine, Pig |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus Cytochrome b245 Light Chain |
| Description | Rabbit Polyclonal |
| Gene | CYBA |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Cytochrome b245 Light Chain antibody (ab75941) at 1 µg/mlLane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 2 : Pancreas (Mouse) Tissue Lysate Lane 3 : Spleen (Mouse) Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab75941 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75941, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa cells at 5µg/ml, and in 4% PFA fixed (10 min) HeLa cells at 5µg/ml.
IHC image of Cytochrome b245 Light Chain staining in Human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab75941, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
NADPH oxidase 4 regulates cardiomyocyte differentiation via redox activation of - NADPH oxidase 4 regulates cardiomyocyte differentiation via redox activation of
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Studies of mitochondrial and nonmitochondrial sources implicate nicotinamide - Studies of mitochondrial and nonmitochondrial sources implicate nicotinamide
Sakellariou GK, Vasilaki A, Palomero J, Kayani A, Zibrik L, McArdle A, Jackson MJ. Antioxid Redox Signal. 2013 Feb 20;18(6):603-21.
Uremia induces abnormal oxygen consumption in tubules and aggravates chronic - Uremia induces abnormal oxygen consumption in tubules and aggravates chronic
Palm F, Nangaku M, Fasching A, Tanaka T, Nordquist L, Hansell P, Kawakami T, Nishijima F, Fujita T. Am J Physiol Renal Physiol. 2010 Aug;299(2):F380-6. doi: