Anti-Cytochrome C antibody
| Name | Anti-Cytochrome C antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab53056 |
| Prices | $392.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ELISA WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide derived from human Cytochrome C |
| Description | Rabbit Polyclonal |
| Gene | CYCS |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Cytochrome C antibody (ab53056) at 1/500 dilutionLane 1 : extracts from Cos7 cells, no immunising peptideLane 2 : extracts from Cos7 cells, immunising peptide present
ICC/IF image of ab53056 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab53056 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
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Martin SA, Hewish M, Sims D, Lord CJ, Ashworth A. Cancer Res. 2011 Mar 1;71(5):1836-48.
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