CYCS

Gene Symbol CYCS
Entrez Gene 54205
Alt Symbol CYC, HCS, THC4
Species Human
Gene Type protein-coding
Description cytochrome c, somatic
Other Description cytochrome c
Swissprots Q6NUR2 P99999 Q96BV4 P00001 Q6NX69 A4D166 B2R4I1
Accessions AAA35732 AAQ96844 AEP27189 EAL24239 EAW93822 EAW93823 EAW93824 P99999 AI365318 AK056360 AK311836 BAG34778 AL713681 CAD28485 AM393213 CAL38091 AM393423 CAL38301 BC005299 AAH05299 BC008475 AAH08475 BC008477 AAH08477 BC009578 AAH09578 BC009579 AAH09579 BC009582 AAH09582 BC009587 AAH09587 BC009602 AAH09602 BC009607 AAH09607 BC014359 AAH14359 BC014361 AAH14361 BC015130 AAH15130 BC016006 AAH16006 BC021994 AAH21994 BC022330 AAH22330 BC024216 BC067222 AAH67222 BC068464 AAH68464 BC070156 AAH70156 BC070346 AAH70346 BC071761 AAH71761 BF446772 BG677560 BT006946 AAP35592 BU597562 CR542175 CAG46972 D00265 BAA00187 DB447825 DQ893038 ABM83964 DQ896279 ABM87278 NM_018947 NP_061820
Function Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
Subcellular Location Mitochondrion intermembrane space. Note=Loosely associated with the inner membrane.
Top Pathways Apoptosis, Non-alcoholic fatty liver disease (NAFLD), p53 signaling pathway, Small cell lung cancer, Legionellosis

Cytochrome c (D18C7) Rabbit mAb (HRP Conjugate) - 12959 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D18C7
Western blot analysis of extracts from various cell lines using Cytochrome c (D18C7) Rabbit mAb (HRP Conjugate).

Cytochrome c (D18C7) Rabbit mAb - 11940 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D18C7
Western blot analysis of extracts from various cell lines using Cytochrome c (D18C7) Rabbit mAb.

Cytochrome c (6H2.B4) Mouse mAb - 12963 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (300 tests)
Host Mouse
Clonality Monoclonal
Clone 6H2.B4
Immunoprecipitation of cytochrome c from RD cell extracts using Mouse (G3A1) mAb IgG1 Isotype Control #5415 (lane 2) or Cytochrome c (6H2.B4) Mouse mAb (lane 3). Lane 1 is 10% input. Western blot was performed using Cytochrome c (D18C7) Rabbit mAb #11940.

Cytochrome c (136F3) Rabbit mAb - 4280 from Cell Signaling Technology

Prices $99.00, $256.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 136F3
Western blot analysis of cell lysates from various cell lines, using Cytochrome c (136F3) Rabbit mAb.

Cytochrome c Antibody - 4272 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western analysis of cell extracts from human (HeLa and Jurkat), monkey (COS), mouse (L929), rat (C6) and purified bovine cytochrome c, using Cytochrome c Antibody.

cytochrome c (6H2) - sc-13561 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
cytochrome c (6H2) Alexa Fluor 647: sc-13561 AF647. Intracellular FCM analysis of fixed and permeabilized K-562 cells. Black line histogram represents the isotype control, normal mouse IgG<sub>1</sub>: sc-24636.

cytochrome c (7H8) - sc-13560 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
cytochrome c (7H8): sc-13560. Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with cytochrome c (7H8), followed by PE-conjugated goat anti-mouse IgG: sc-3738. Black line histogram represents the isotype control, normal mouse IgG2b: sc-3879.

cytochrome c (A-8) - sc-13156 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
cytochrome c (A-8): sc-13156. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human pancreas tumor showing cytoplasmic staining.

cytochrome c (C-20) - sc-8385 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
cytochrome c (C-20): sc-8385. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing cytoplasmic staining.

cytochrome c (H-104) - sc-7159 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
cytochrome c (H-104): sc-7159. Western blot analysis of cytochrome c expression in HL-60 (A), K-562 (B) andPC-3 (C) whole cell lysates.

cytochrome c (2G8) - sc-65396 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
cytochrome c (2G8): sc-65396. Western blot analysis of cytochrome c expression in HeLa whole cell lysate.

Anti-Cytochrome C antibody - ab53056 from Abcam

Prices $392.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Cytochrome C antibody (ab53056) at 1/500 dilutionLane 1 : extracts from Cos7 cells, no immunising peptideLane 2 : extracts from Cos7 cells, immunising peptide present

Anti-Cytochrome C antibody - ab21465 from Abcam

Prices $359.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-Cytochrome C antibody - ab31103 from Abcam

Prices $376.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal
ab31103 at a 1/50 dilution, staining human Cytochrome C in human brain tissue by Immunohistochemistry, Formalin fixed Paraffin embedded tissue.

Anti-Cytochrome C antibody [2CYTC-199] - ab50050 from Abcam

Prices $385.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2CYTC-199
ICC/IF image of ab50050 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab50050, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Cytochrome C antibody [7H8.2C12] - ab13575 from Abcam

Prices $400.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 7H8.2C12
Ab13575 staining human normal skin tissue. Staining is localised to mitochondria.Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-Cytochrome C antibody [EP1326-80-5] - ab76107 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1326-80-5
ab76107 at 1/100 dilution staining Cytochrome C in human kidney by Immunohistochemistry using paraffin-embedded tissue.

Anti-Cytochrome C antibody [EP1326-80-5-4] - ab76237 from Abcam

Prices $391.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1326-80-5-4
Anti-Cytochrome C antibody [EP1326-80-5-4] (ab76237) at 1/500 dilution + HeLa cell lysate at 10 µgSecondarygoat anti-rabbit HRP at 1/1000 dilutionObserved band size : 14 kDa (why is the actual band size different from the predicted?)

Anti-Cytochrome C antibody [EPR1327] - ab133504 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1327
Anti-Cytochrome C antibody [EPR1327] (ab133504) at 1/5000 dilution (purified) + Human fetal kidney tissue lysate at 10 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Anti-Cytochrome C antibody [EPR1327] (Alexa Fluor® 488) - ab192485 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1327
Overlay histogram showing HeLa cells stained with ab192485 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab192485, 1/50 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-Cytochrome C antibody [EPR1327] (Alexa Fluor® 647) - ab192486 from Abcam

Prices $433.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1327
ab192486 staining Cytochrome C in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab192486 at a working dilution of 1 in 50 (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 2µg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.This product also gave a positive signal in 100% methanol (5 min) fixed HeLa cells under the same testing conditions.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-Cytochrome C antibody [EPR1327] (HRP) - ab193239 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1327
All lanes : Anti-Cytochrome C antibody [EPR1327] (HRP) (ab193239) at 1/5000 dilutionLane 1 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell LysateLane 2 : Heart (Human) Tissue Lysate - adult normal tissueLane 3 : Kidney (Human) Tissue Lysate - adult normal tissueLysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

Anti-Cytochrome C antibody, prediluted - ab31104 from Abcam

Prices $376.00
Sizes 7 ml
Host Rabbit
Clonality Polyclonal
ab31104 staining human Cytochrome C in human brain tissue by Immunohistochemistry, Formalin fixed Paraffin embedded tissue

Anti-Cytochrome C antibody [SJL2-4(apo)] - ab16076 from Abcam

Prices $372.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone SJL2-4(apo)

Anti-CYCS / Cytochrome c Antibody - LS-C18635 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
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