Anti-Cytochrome C Oxidase subunit VIb antibody [3F9D3D11AF6]

Name	Anti-Cytochrome C Oxidase subunit VIb antibody [3F9D3D11AF6]
Supplier	Abcam
Catalog	ab110266
Prices	$382.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone	3F9D3D11AF6
Applications	ICC/IF ICC/IF WB
Species Reactivities	Mouse, Rat, Bovine, Human
Antigen	Bovine Complex IV (native holoenzyme protein, purified from heart)
Description	Mouse Monoclonal
Gene	COX6B1
Conjugate	Unconjugated
Supplier Page	Shop

Product images

ICC/IF image of ab110266 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110266, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

All lanes : Anti-Cytochrome C Oxidase subunit VIb antibody [3F9D3D11AF6] (ab110266) at 1 µg/mlLane 1 : Isolated mitochondria from Human Heart at 5 µgLane 2 : Isolated mitochondria from Bovine Heart at 4 µgLane 3 : Isolated mitochondria from Rat Heart at 10 µgLane 4 : Isolated mitochondria from Mouse Heart at 10 µg

Product References

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