COX6B1

Gene Symbol COX6B1
Entrez Gene 100270792
Alt Symbol COX6B
Species Bovine
Gene Type protein-coding
Description cytochrome c oxidase subunit VIb polypeptide 1 (ubiquitous)
Other Description COX VIb-1|Cytochrome c oxidase polypeptide VII|Cytochrome c oxidase subunit AED|cytochrome c oxidase subunit 6B1
Swissprots Q54A32 P00429
Accessions DAA19887 P00429 AB098750 BC102366 AAI02367 X15112 CAA33211 NM_176675 NP_788848
Function Connects the two COX monomers into the physiological dimeric form.
Subcellular Location Mitochondrion intermembrane space.
Top Pathways Alzheimer's disease, Huntington's disease, Cardiac muscle contraction, Non-alcoholic fatty liver disease (NAFLD), Oxidative phosphorylation

Anti-Cytochrome C Oxidase subunit VIb antibody [3F9D3D11AF6] - ab110266 from Abcam

Prices $382.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3F9D3D11AF6
ICC/IF image of ab110266 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110266, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Cytochrome C Oxidase subunit VIb antibody [3F9D3D11AF6] (Alexa Fluor® 488) - ab198315 from Abcam

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3F9D3D11AF6
ab198315 staining Cytochrome C Oxidase subunit VIb in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198315 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-Cytochrome C Oxidase subunit VIb antibody [3F9D3D11AF6] (HRP) - ab198317 from Abcam

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3F9D3D11AF6
Anti-Cytochrome C Oxidase subunit VIb antibody [3F9D3D11AF6] (HRP) (ab198317) at 1/5000 dilution + Human Heart Mitochondrial lysate (ab110337) at 5 µgdeveloped using the ECL techniquePerformed under reducing conditions.
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