Flow cytometric analysis of 2% paraformaldehyde-fixed K562 cells labeling Cytochrome P450 26B with ab182162 at 1/120 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Anti-Cytochrome P450 26B antibody [EPR13182] (ab182162) at 1/10000 dilution + K562 cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
All lanes : Anti-Cytochrome P450 26B antibody [EPR13182] (ab182162) at 1/5000 dilutionLane 1 : HeLa cell lysateLane 2 : BxPC-3 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution