ab14404 staining Cytokeratin 1 in Human LNCaP, prostate cancer cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 1% Donkey serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/100 in 1% Donkey serum in PBST) for 1 hour at 22°C. A DyLight® 594-conjugated Donkey anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Red - cytokeratin 1, Green - Golgi marker ST3Gal1, Blue - nucleus (DAPI). See Abreview
Anti-Cytokeratin 1 antibody (ab93652) at 1/500 dilution + fetal skin lysate
ab93652, at a 1/100 dilution, staining Cytokeratin 1 in the membrane of formalin fixed, paraffin embedded skin tissue section by Immunohistochemistry.