Western blot analysis of extracts from COS cells, untransfected (lane 1), or transfected with wild-type ASK1 (lanes 2,3), using Phospho-ASK1 (Thr845) Antibody (upper) or ASK1 Antibody #3762 (lower). In lane 3, the lysate was treated with lamda phosphatase to demonstrate phospho-specificity of Phospho-ASK1 (Thr845) Antibody.
Western blot analysis of extracts from HEK293 cells, untransfected (lane 1) or transfected with wild-type ASK1 (lane 2) or T845A mutant ASK1 (lane 3), using Phospho-ASK1 (Thr845) Antibody (upper) or ASK1 Antibody #3762 (lower). (Lysates were provided by Dr. Wang Min, Dept. of Pathology, Yale University, New Haven, CT).