MAP3K5

Gene Symbol MAP3K5
Entrez Gene 4217
Alt Symbol ASK1, MAPKKK5, MEKK5
Species Human
Gene Type protein-coding
Description mitogen-activated protein kinase kinase kinase 5
Other Description ASK-1|MAP/ERK kinase kinase 5|MAPK/ERK kinase kinase 5|MEK kinase 5|MEKK 5|apoptosis signal regulating kinase 1|apoptosis signal-regulating kinase 1
Swissprots B4DGB2 Q5THN3 Q99683 A6NIA0 Q99461
Accessions AAT52164 EAW47942 Q99683 AB209093 BAD92330 AK293953 BAG57328 AK294507 BAG57723 AK310942 BC054503 AAH54503 BC088829 AAH88829 D84476 BAA12684 U67156 AAC50894 XM_011535837 XP_011534139 XM_011535838 XP_011534140 XM_011535839 XP_011534141 NM_005923 NP_005914
Function Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7
Subcellular Location Cytoplasm. Endoplasmic reticulum. Note=Interaction with 14-3-3 proteins alters the distribution of MAP3K5/ASK1 and restricts it to the perinuclear endoplasmic reticulum region.
Tissue Specificity Abundantly expressed in heart and pancreas.
Top Pathways Amyotrophic lateral sclerosis (ALS), MAPK signaling pathway, Neurotrophin signaling pathway, Protein processing in endoplasmic reticulum, TNF signaling pathway

Phospho-ASK1 (Ser83) Antibody - 3761 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from NIH/3T3 cells, untransfected (lanes 1 and 4) or transfected wild type (lanes 2 and 5) or Ser83Ala mutant (lanes 3 and 6) ASK1, using Phospho-ASK1 (Ser83) Antibody (A and B) or ASK1 Antibody #3762 (C and D). The phospho-specificity of the antibody was further characterized by treating the membranes (B and D) with calf intestinal alkaline phosphatase (CIP) after Western transfer.

Phospho-ASK1 (Thr845) Antibody - 3765 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from COS cells, untransfected (lane 1), or transfected with wild-type ASK1 (lanes 2,3), using Phospho-ASK1 (Thr845) Antibody (upper) or ASK1 Antibody #3762 (lower). In lane 3, the lysate was treated with lamda phosphatase to demonstrate phospho-specificity of Phospho-ASK1 (Thr845) Antibody.

Phospho-ASK1 (Ser967) Antibody - 3764 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from COS7 cells transfected with wild-type or Ser967Ala mutant ASK1, using Phospho-ASK1 (Ser967) Antibody (A,B) or ASK1 Antibody #3762 (C,D). The phospho-specificity of the antibody was further characterized by treating the membrane with calf intestinal alkaline phosphatase (CIP) after Western transfer (B,D).

ASK1 (D11C9) Rabbit mAb - 8662 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D11C9
Western blot analysis of extracts from various cell lines using ASK1 (D11C9) Rabbit mAb (upper) or Pan-Actin (D18C11) Rabbit mAb #8456 (lower).

ASK1 Antibody - 3762 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from NIH/3T3 cells, untransfected (lane 1) or transfected with wild type (lane 2) or Ser83Ala mutant (lane 3) ASK1, using ASK1 Antibody.

ASK 1 (F-9) - sc-5294 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ASK 1 (F-9): sc-5294. Western blot analysis of ASK 1 expression in HeLa (A) and BJAB (B) whole cell lysates.

ASK 1 (H-2) - sc-390275 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ASK 1 (H-2): sc-390275. Western blot analysis of ASK 1 expression in non-transfected: sc-117752 (A) and human ASK 1 transfected: sc-116417 (B) 293T whole cell lysates.

ASK 1 (E-6) - sc-390397 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ASK 1 (E-6): sc-390397. Western blot analysis of ASK 1 expression in non-transfected: sc-117752 (A) and human ASK 1 transfected: sc-116417 (B) 293T whole cell lysates.

ASK 1 (H-300) - sc-7931 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
ASK 1 (H-300): sc-7931. Western blot analysis of ASK 1 expression in BJAB whole cell lysate.

ASK 1 (L-20) - sc-49371 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ASK 1 (L-20): sc-49371. Western blot analysis of ASK 1 expression in mouse liver tissue extract.

ASK 1 (N-19) - sc-6368 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ASK 1 (N-19): sc-6368. Western blot analysis of ASK 1 expression in non-transfected: sc-117752 (A) and human ASK 1 transfected: sc-116417 (B) 293T whole cell lysates.

p-ASK 1 (B-5) - sc-166967 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of ASK 1 phosphorylation in non-transfected: sc-117752 (A,D), untreated human ASK 1 transfected: sc-116417 (B,E) and lambda protein phosphatase treated human ASK 1 transfected: sc-116417 (C,F) 293T whole cell lysates. Antibodies tested include p-ASK 1 (B-5): sc-166967 (A,B,C) and ASK 1 (H-300): sc-7931 (D,E,F).

p-ASK 1 (Ser 83) - sc-33362 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
p-ASK 1 (Ser 83): sc-33362. Western blot analysis of ASK 1 phosphorylation in non-transfected: sc-117752 (A) and human ASK 1 transfected: sc-111456 (B) 293T whole cell lysates.

p-ASK 1 (Ser 966) - sc-101634 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-ASK 1 (Ser 966): sc-101634. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue extract showing cytoplasmic staining.

p-ASK 1 (Ser 83) - sc-101633 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-ASK 1 (Ser 83): sc-101633. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue extract showing cytoplasmic staining.

Anti-ASK1 (phospho S83) antibody - ab47304 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab47304 staining human breast carcinoma tissue by IHC-P (left hand panel). The right hand panel shows staining in the presence of phospho-peptide.

Anti-ASK1 (phospho S83) antibody - Azide free - ab14380 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-ASK1 (phospho S966) antibody - ab195821 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-ASK1 (phospho S966) antibody (ab195821) at 1/500 dilutionLane 1 : TNF treated 293 whole cell lysates with antigen-specific peptideLane 2 : TNF treated 293 whole cell lysatesLysates/proteins at 12.5 µg per lane.

Anti-ASK1 (phospho S966) antibody - ab39402 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab39402, at a 1/100 dilution, staining human ASK1 in breast carcinoma tissue by Immunohistochemistry, Paraffin embedded tissue.

Anti-ASK1 antibody - ab131506 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence analysis of methanol fixed HeLa cells labelled with ab131506 at 1/100 dilution.

Anti-ASK1 antibody - ab2004 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-ASK1 antibody (ab2004) at 1/500 dilution + SW1351 whole cell lysate

Anti-ASK1 antibody - ab62575 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab62575 at 2µg/ml staining ASK1 in A431 cells by Immunocytochemistry.

Anti-ASK1 antibody - ab131358 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-ASK1 antibody (ab131358) at 1/500 dilutionLane 1 : MDA-MB 435 cell extractLane 2 : MDA-MB 435 cell extract with blocking peptide

Anti-ASK1 antibody - ab39639 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-ASK1 antibody (ab39639) at 1/500 dilutionLane 1 : MDA-MB-435 cell extracts Lane 2 : MDA-MB-435 cell extracts with synthesized peptide (peptide preincubated with ab39639)

Anti-ASK1 antibody - ab39650 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab39650, at 1/50 dilution, staining ASK1 in Human breast carcinoma tissue by Immunohistochemistry, Paraffin embedded tissue. Left: sample not treated with peptide. Right: sample treated with synthetic peptide.
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