Anti-Cytokeratin 17 antibody

• Supplier: Abcam
• Catalog: ab53707
• Prices: $385.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF WB IHC-P ELISA ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide derived from human Cytokeratin 17
• Description: Rabbit Polyclonal
• Gene: KRT17
• Conjugate: Unconjugated

Product images

ab53707 at 1/50 dilution staining Cytokeratin 17 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.

All lanes : Anti-Cytokeratin 17 antibody (ab53707) at 1/500 dilutionLane 1 : HuvEc cell extractLane 2 : HuvEc cell extract with immunising peptide

ICC/IF image of ab53707 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53707, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

A novel role for keratin 17 in coordinating oncogenic transformation and cellular - A novel role for keratin 17 in coordinating oncogenic transformation and cellular

Sankar S, Tanner JM, Bell R, Chaturvedi A, Randall RL, Beckerle MC, Lessnick SL. Mol Cell Biol. 2013 Nov;33(22):4448-60.

Micro-RNA-31 controls hair cycle-associated changes in gene expression programs - Micro-RNA-31 controls hair cycle-associated changes in gene expression programs

Mardaryev AN, Ahmed MI, Vlahov NV, Fessing MY, Gill JH, Sharov AA, Botchkareva NV. FASEB J. 2010 Oct;24(10):3869-81.