Anti-Cytokeratin 17 antibody [CK-E3]
| Name | Anti-Cytokeratin 17 antibody [CK-E3] |
|---|---|
| Supplier | Abcam |
| Catalog | ab49749 |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone | CK-E3 |
| Applications | WB DB ICC/IF ICC/IF IHC-F |
| Species Reactivities | Rat, Goat, Bovine, Human, Pig |
| Antigen | A cytoskeletal preparation from Rat colon epithelium |
| Description | Mouse Monoclonal |
| Gene | KRT17 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab49749 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49749, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Basal-cell keratins in cervical reserve cells and a comparison to their - Basal-cell keratins in cervical reserve cells and a comparison to their
Smedts F, Ramaekers F, Troyanovsky S, Pruszczynski M, Robben H, Lane B, Leigh I, Plantema F, Vooijs P. Am J Pathol. 1992 Mar;140(3):601-12.
Patterns of expression of keratin 17 in human epithelia: dependency on cell - Patterns of expression of keratin 17 in human epithelia: dependency on cell
Troyanovsky SM, Guelstein VI, Tchipysheva TA, Krutovskikh VA, Bannikov GA. J Cell Sci. 1989 Jul;93 ( Pt 3):419-26.
Monoclonal antibody mapping of keratins 8 and 17 and of vimentin in normal human - Monoclonal antibody mapping of keratins 8 and 17 and of vimentin in normal human
Guelstein VI, Tchypysheva TA, Ermilova VD, Litvinova LV, Troyanovsky SM, Bannikov GA. Int J Cancer. 1988 Aug 15;42(2):147-53.