Anti-Cytokeratin 8 + 18 + 19 antibody [2A4]
| Name | Anti-Cytokeratin 8 + 18 + 19 antibody [2A4] |
|---|---|
| Supplier | Abcam |
| Catalog | ab41825 |
| Prices | $390.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 2A4 |
| Applications | WB ICC/IF ICC/IF IHC-P IHC-F FC |
| Species Reactivities | Rat, Human, Pig |
| Antigen | Cytokeratins isolated from cultured pig kidney epithelial cells |
| Description | Mouse Monoclonal |
| Gene | KRT8 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of ab41825 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab41825, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
![Overlay histogram showing MCF7 cells stained with ab41825 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41825, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/5868_Cytokeratin-8-18-19-Primary-antibodies-ab41825-2.jpg)
Overlay histogram showing MCF7 cells stained with ab41825 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41825, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
ab41825 staining Cytokeratin 8 + 18 + 19 in the MCF-7 epithelial cell line from Human mammary glands by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Paraformaldehyde, permeabilized with Triton X-100 0.3% in PBS and blocked with 5% serum for 8 hours at 4°C. Samples were incubated with primary antibody (10ug/ml) for 8 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal(10ug/ml) was used as the secondary antibody.See Abreview
Product References
Bioengineered 3D human kidney tissue, a platform for the determination of - Bioengineered 3D human kidney tissue, a platform for the determination of
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Flexible micro spring array device for high-throughput enrichment of viable - Flexible micro spring array device for high-throughput enrichment of viable
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Tubular cell-enriched subpopulation of primary renal cells improves survival and - Tubular cell-enriched subpopulation of primary renal cells improves survival and
Kelley R, Werdin ES, Bruce AT, Choudhury S, Wallace SM, Ilagan RM, Cox BR, Tatsumi-Ficht P, Rivera EA, Spencer T, Rapoport HS, Wagner BJ, Guthrie K, Jayo MJ, Bertram TA, Presnell SC. Am J Physiol Renal Physiol. 2010 Nov;299(5):F1026-39. doi: