Anti-Cytokeratin 8 (phospho S73) antibody [E431-2]
| Name | Anti-Cytokeratin 8 (phospho S73) antibody [E431-2] |
|---|---|
| Supplier | Abcam |
| Catalog | ab32579 |
| Prices | $357.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | E431-2 |
| Applications | IHC-F WB ICC/IF ICC/IF IHC-P |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Full length native protein (purified) corresponding to Human Cytokeratin 8 |
| Description | Rabbit Monoclonal |
| Gene | KRT8 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunofluorescent staining of A431 cells using anti-Phospho-Keratin 8 (ab32579) diluted 1/250.
IHC-P analysis (with antigen retrieval) of liver carcinoma using anti-Phospho-Keratin 8 (ab32579) diluted 1/500.
Ab32579 staining cells from a human pancreatic cell line by ICC/IF. The cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 1% BSA for 30 minutes at 22°C. Ab32579 was diluted 1/25 and incubated with the sample for 1 hour at 22°C. An Alexa Fluor® 488 conjugated donkey anti-rabbit antibody was used as the secondary.See Abreview
Immunohistochemical analysis of Mouse lung adenoma, staining Cytokeratin 8 (phospho S73) with ab32579.Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/1000 in PBS) for 8 hours at 4°C. A biotin-conjugated goat anti-rabbit polyclonal IgG (1/1000) was used as the secondary antibody.See Abreview
Product References
A novel mechanism of keratin cytoskeleton organization through casein kinase - A novel mechanism of keratin cytoskeleton organization through casein kinase
Kuga T, Kume H, Kawasaki N, Sato M, Adachi J, Shiromizu T, Hoshino I, Nishimori T, Matsubara H, Tomonaga T. J Cell Sci. 2013 Oct 15;126(Pt 20):4721-31.
Autophagy regulates keratin 8 homeostasis in mammary epithelial cells and in - Autophagy regulates keratin 8 homeostasis in mammary epithelial cells and in
Kongara S, Kravchuk O, Teplova I, Lozy F, Schulte J, Moore D, Barnard N, Neumann CA, White E, Karantza V. Mol Cancer Res. 2010 Jun;8(6):873-84.
Evidence for cross-reactivity of JAM-C antibodies: implications for cellular - Evidence for cross-reactivity of JAM-C antibodies: implications for cellular
Betanzos A, Schnoor M, Severson EA, Liang TW, Parkos CA. Biol Cell. 2009 Jun 4;101(8):441-53.
Cytokeratin 8/18 overexpression and complex formation as an indicator of GST-P - Cytokeratin 8/18 overexpression and complex formation as an indicator of GST-P
Kakehashi A, Inoue M, Wei M, Fukushima S, Wanibuchi H. Toxicol Appl Pharmacol. 2009 Jul 1;238(1):71-9.
Bispecific and human disease-related anti-keratin rabbit monoclonal antibodies. - Bispecific and human disease-related anti-keratin rabbit monoclonal antibodies.
Tao GZ, Nakamichi I, Ku NO, Wang J, Frolkis M, Gong X, Zhu W, Pytela R, Omary MB. Exp Cell Res. 2006 Feb 15;312(4):411-22. Epub 2005 Dec 15.