Anti-Cytokeratin antibody [MNF116]
| Name | Anti-Cytokeratin antibody [MNF116] |
|---|---|
| Supplier | Abcam |
| Catalog | ab756 |
| Prices | $378.00 |
| Sizes | 500 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | MNF116 |
| Applications | IHC-P IHC-F FC |
| Species Reactivities | Human |
| Antigen | Crude extract of splenic cells from a nude mouse engrafted with MCF-7 cells |
| Description | Mouse Monoclonal |
| Gene | KRT1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Formalin fixed paraffin embedded human lung carcinoma stained with Cytokeratin using ABC and AEC chromogen.
ab756 staining human tonsil by IHC-P.
![Overlay histogram showing MCF7 cells stained with ab756 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab756, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/6000_Cytokeratin-Primary-antibodies-ab756-1.jpg)
Overlay histogram showing MCF7 cells stained with ab756 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab756, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Product References
Differences in replication kinetics and cell tropism between neurovirulent and - Differences in replication kinetics and cell tropism between neurovirulent and
Gryspeerdt AC, Vandekerckhove AP, Garre B, Barbe F, Van de Walle GR, Nauwynck HJ. Vet Microbiol. 2010 May 19;142(3-4):242-53.
Cytokeratin-specific monoclonal antibodies are reactive with tumours of smooth - Cytokeratin-specific monoclonal antibodies are reactive with tumours of smooth
Norton AJ, Thomas JA, Isaacson PG. Histopathology. 1987 May;11(5):487-99.