Anti-DACH1 antibody [EPR2841Y]
| Name | Anti-DACH1 antibody [EPR2841Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab77234 |
| Prices | $357.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EPR2841Y |
| Applications | WB IP FC |
| Species Reactivities | Mouse, Human |
| Antigen | A synthetic peptide corresponding to residues near the C terminus of human DACH1 |
| Description | Rabbit Monoclonal |
| Gene | DACH1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-DACH1 antibody [EPR2841Y] (ab77234) at 1/1000 dilutionLane 1 : MCF-7 cell lysateLane 2 : Hela cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/6151_ab77234.jpg)
All lanes : Anti-DACH1 antibody [EPR2841Y] (ab77234) at 1/1000 dilutionLane 1 : MCF-7 cell lysateLane 2 : Hela cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution
Overlay histogram showing HeLa cells stained with ab77234 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77234, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.