IHC image of Dact2 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab42099, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
All lanes : Anti-Dact2 antibody (ab42099) at 1 µg/mlLane 1 : Pancreas (Mouse) Tissue Lysate (ab29363)Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)Lane 3 : Thymus (Mouse) Tissue Lysate - normal tissue (ab29285)Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
All lanes : Anti-Dact2 antibody (ab42099) at 1 µg/mlLane 1 : Kidney (Mouse) Tissue LysateLane 2 : Brain (Mouse) Tissue LysateLane 3 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)Lane 4 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 6 : SK N SH (Human neuroblastoma) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilutionPerformed under reducing conditions.